高效液相色谱法测定海洛因依赖者尿液中的吗啡

目的:建立高效液相色谱法测定海洛因依赖者尿液中的吗啡浓度。方法:尿样经酸水解后用乙酸乙酯提取,然后采用HPLC分析。仪器条件:Diamonsil(tm)钻石C18色谱柱,流动相0·012mol·L-1磷酸二氢钾-乙腈(90:10,含0·05mmol·L-1三乙胺),流速1·0ml·min-1,检测波长205nm。结果:吗啡和内标与内源性杂质分离良好;在0·050-2·000μg·ml-1浓度范围线性关系良好(r=0·9999);检测限为0·020μg·ml-1;高、中、低3种浓度相对回收率为95·3%-104·6%,日内变异(RSD)为4·0%-8·1%,日间变异为1·9%-10·7%。结论:本法简便、准确,可用于测定海洛因依赖者尿液中的吗啡浓度。

DETERMINATION OF MORPHINE IN THE URINE OF HEROIN ABUSERS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Objective: To develop a high performance liquid chromatographic method with UV detection for the determination of morphine in the urine of heroin abusers. Methods: Morphine was extracted from the urine by ethyl acetate one step extraction method after acid hydrolysis, then determined by HPLC with Diamonsil(tm) C_ 18 column. The mobile phase consisted of 0.012 mol·L~ -1 KH_2PO_4-acetonitrile (90:10) containing 0.5 mmol·L~ -1 triethylamine. The flow-rate was 1.0 ml·min~ -1, and the wavelength of UV detection was 205 nm. Results: Morphine and internal standard could be separated from endogenous compounds. Linearity was verified from 0.050-2.000 μg·ml~ -1 (r=0.9999) and the limit of quantitation (LOQ) was 0.020 μg·ml~ -1. The relative recovery of morphine at three different concentration levels ranged from 95.3 % to 104.6%; the RSD value of within-day was 4.0%-8.1% and the value of between-day was 1.9%-10.7%, respectively. Conclusion: The method can quantify morphine in the urine of heroin abusers rapidly and accurately.