The influence of platelet-rich fibrin on angiogenesis in guided bone regeneration using xenogenic bone substitutes: A study of rabbit cranial defects |
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| Affiliation: | 1. Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China;2. Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China;3. Key laboratory of Stomatology, Fujian Province University, Fuzhou 350002, China;4. University of Chinese Academy of Sciences, Beijing 1000049, China;5. Department of Stomatology, Fujian Provincial Geriatric Hospital, Fuzhou 350002, China;1. Department of Emergency Medicine, E-DA Hospital, I-Shou University, Kaohsiung, Taiwan;2. Division of Cardiology, Department of Internal Medicine, Taiwan;3. Center for Translational Research in Biomedical Sciences, Taiwan;4. Basic Science, Nursing Department, Meiho Institute of Technology, Pingtung, Taiwan;5. Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan;6. Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan |
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| Abstract: | PurposeThe purpose of this study was to investigate the influence of platelet-rich fibrin (PRF) on angiogenesis and osteogenesis in guided bone regeneration (GBR) using xenogenic bone in rabbit cranial defects.Materials and methodsIn each rabbit, 2 circular bone defects, one on either side of the midline, were prepared using a reamer drill. Each of the experimental sites received bovine bone with PRF, and each of the control sites received bovine bone alone. The animals were sacrificed at 1 week (n = 4), 2 weeks (n = 3) and 4 weeks (n = 3). Biopsy samples were examined histomorphometrically by light microscopy, and expression of vascular endothelial growth factor (VEGF) was determined by immunohistochemical staining.ResultsAt all experimental time points, immunostaining intensity for VEGF was consistently higher in the experimental group than in the control group. However, the differences between the control group and the experimental group were not statistically significant in the histomorphometrical and immunohistochemical examinations.ConclusionsThe results of this study suggest that PRF may increase the number of marrow cells. However, PRF along with xenogenic bone substitutes does not show a significant effect on bony regeneration. Further large-scale studies are needed to confirm our results. |
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| Keywords: | PRF (platelet-rich fibrin) Vascular endothelial growth factor Non-organic bovine bone |
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