治疗尿毒症新型血液灌流吸附剂体外实验研究

目的:研制用于治疗尿毒症的新型血液灌流吸附剂，通过与已上市血液灌流产品对照研究探讨其对肌酐、尿酸和β2微球蛋白的清除效果及血液相容性。方法:通过体外血浆吸附实验研究血液灌流吸附剂对肌酐、尿酸和β2微球蛋白的清除效率，通过血浆蛋白吸附性、电解质吸附性、血细胞吸附性、溶血性以及凝血性实验评价血液灌流吸附剂的血液相容性。结果:新型HB-H-10血液灌流吸附剂对肌酐、尿酸和β2微球蛋白的吸附率分别达（40.5±2.3）%、（24.2±1.8）%和（46.9±1.3）%，与其他吸附剂相比，吸附效果具有统计学意义（P<0.05）；该吸附剂对血浆蛋白总蛋白、白蛋白、球蛋白、白细胞、红细胞和血小板吸附率分别为（5.69±0.32）%、（4.76±0.42）%、（7.33±0.33）%、（2.88±0.32）%、（9.22±0.26）%和（11.98±0.43）%，与其他吸附剂相比，吸附效果具有统计学意义（P<0.05）；该吸附剂对血浆电解质、溶血率、凝血影响无统计学意义（均P>0.05）,具有优异的血液相容性。结论:研制的新型HB-H-10吸附剂可作为血液灌流吸附剂治疗尿毒症，达到该产品更新换代和升级的效果。

In vitro experimental study on a new hemoperfusionadsorbent to cure uremia

Objective: To develop a new kind of hemoperfusion adsorbent to cure uremia，and study the clearance effect on creatinine，uric acid，β2-microglobulin and hemocompatibility by comparing with the clinical hemoperfusion products. Methods: The clearance efficiency of hemoperfusion adsorbents on creatinine，uric acid and β2-microglobulin were studied by plasma adsorption experiment in vitro. The hemocompatibilities were evaluated by plasma protein adsorption，electrolyte adsorption，blood cell adsorption，hemolysis rate and coagulation experiment. Results: The clearance efficiency of HB-H-10 hemoperfusion adsorbent on creatinine，uric acid and β2-microglobulin were（40.5±2.3）%,（24.2±1.8）% and （46.9±1.3）%.The clearance efficiency was statistically significant compared with other adsorbents（P<0.05）. The adsorption rates of HB-H-10 hemoperfusion adsorbent on total protein，albumin，globulin，white blood cells，red blood cells and platelets were（5.69±0.32）%，（4.76±0.42）%，（7.33±0.33）%，（2.88±0.32）%，（9.22±0.26）% and （11.98±0.43）%. Compared with the other adsorbents，the adsorption rates of HB-H-10 hemoperfusion adsorbent were statistically significant （P<0.05）. The adsorbent had no significant effect on plasma electrolyte，hemolysis rate and coagulation（all P>0.05）. It had excellent hemocompatibility. Conclusion: The new HB-H-10 adsorbent is expected to be used as hemoperfusion adsorbent in the treatment of uremia. It can achieve the effect of updating and upgrading the presented products.