乳腺癌超声造影指标与临床病理及肿瘤相关成纤维细胞数量的相关性

目的分析乳腺癌超声造影指标与临床病理的关联性，探索与超声造影指标相关性较强的病理因子与肿瘤相关成纤维细胞(TAF)相关性的数量分布特点。方法纳入119例乳腺癌患者，采用超声造影的手段，记录乳腺癌超声造影特征，将乳腺癌肿块的活跃边缘带及病灶旁正常带进行标记，采用免疫组化对靶细胞进行标记，以及形态学的特征来确认TAF细胞，并进行统计分析。结果当乳腺癌肿块>2 cm、组织学分级高、临床分期早时，超声造影后肿块边界增大，呈高增强，呈现灌注缺损、出现穿支血流几率高(P < 0.05)。超声造影后呈现充盈缺损状态，癌旁正常带的TAF细胞数量较多(P < 0.05)。造影后出现高增强，在活跃边缘带的TAF细胞数量多(P < 0.05)。当病灶较大时，活跃边缘带TAF数量较多(P < 0.05)。当临床分期早、组织学分级高时，两个区域的TAF数量较多(P < 0.05)。而乳腺癌造影后病灶的增强范围与病灶活跃边缘带及癌旁正常带的TAF数量均无关联(P> 0.05)。超声造影后的充盈缺损状态与边缘活跃带的TAF的数量无关联(P>0.05)，且病灶的造影增强程度与癌旁的正常带的TAF数量无关(P>0.05)。结论TAF是乳腺癌预后不良的因素，TAF的数量在一定程度上反映乳腺癌的分化和转移力。 

Correlation between contrast-enhanced ultrasound and clinicopathological indicators and TAF in breast cancer

ObjectiveTo analyze the correlation between clinic-pathological and CEUS indicators in breast cancer anf explored the correlation between factors with good correlation with CEUS qualitative indicators and the characteristics of TAF in breast cancer.MethodsA total of 119 breast cancer patients were included in the study. CEUS was used to record the characteristics of CEUS. Marked the active marginal band of breast cancer mass and the normal band beside the lesion and marked the study section of mass. Immunohistochemical examination was performed. Target cells were labeled and combined with morphological characteristics to differentiate TAF cells. SPSS22.0 was adopted for statistical analysis.ResultsWhen the mass of breast cancer was more than 2 cm, the histological grade and clinical stage were higher, and the mass range, high enhancement, the probability of perfusion defect and perforator blood flow in contrast-enhanced were higher (P < 0.05). If there was filling defect, the distribution of TAF in the normal adjacent zone increased (P < 0.05). If there was high enhancement after mammography, the distribution of TAF in the active marginal zone of breast cancer increased (P < 0.05). When the lesions were large, TAF in the active marginal zone was more distributed (P < 0.05). When the clinical stage and histological grade were higher, the TAF of the two regions were higher (P < 0.05). But there was no correlation between the enhancement range and the distribution of TAF in the active marginal zone and the normal adjacent zone (P>0.05). There was no correlation between the occurrence of filling defect and the distribution of TAF in the active marginal zone (P>0.05). So the degree of contrast had nothing to do with the distribution of TAF in the normal zone (P>0.05).ConclusionTAF is a factor for poor prognosis of breast cancer. The number of TAF reflected the differentiation and metastasis of breast cancer to a certain extent.