狭小空间法构建抑郁症大鼠模型的优劣探索

目的 采用狭小空间法构建抑郁症大鼠模型(M),检测抑郁症大鼠的生理指标、行为变化及海马区的改变;将其与常规抑郁模型——慢性温和不预知应激(CUMS)抑郁症大鼠的相应指标进行差异性比较;亟望为抑郁症大鼠模型构建提供一种新方法.方法 取30只大鼠将其随机分成3组:(1)M模型抑郁症大鼠(M组):采用10 cm×10 cm×...

The Advantages and disadvantages of constructing depression rat model with narrow space method

Objective The depression rat model (M) was established using narrow space method; and the physiological indexes, behavioral changes and hippocampal changes of the depressed rats were assessed. These indicators were compared between the established model and the conventional depression model - chronic unpredictable mild stress (CUMS) depression rats. So thus to provide a new method for the construction of rat model of depression. Methods Thirty rats were randomly divided into 3 groups, including Group M, Group CUMS, and Group Control. In Group M, rats were kept in solitary cage of 10 cm*10 cm*5 cm, which simulated narrow space, for 4 weeks. In Group CUMS, rats were individually placed in acrylic cages for 4 weeks to induce social isolation; at the same time, 4 cycles of weekly stress regimes were administered, consisting of the following stressors: 30°cage tilt (overnight), food deprivation (24 h), cold swimming (5-min at 4℃), tail pinch (1-min), moist bedding (8 h) and overnight stroboscopic lighting. The stressors were administered daily in a random order for 4 consecutive weeks. In Group Control, rats were routinely raised for 4 weeks. The behavioral changes were compared, including body mass, water intake, sugar water preference test, open-field test. HE staining was used to observe the morphological changes of hippocampus. The content of 5-hydroxytryptamine (5-HT) in the hippocampus was assessed by ELISA. Results Compared with Group Control, the body mass and sugar water preference in the Group M were significantly different after 14 days (P<0.05); and the water intake, food intake, upright times and crossing times between squares in open field test were significantly different after 7 days (P<0.05). There was significant difference in the body mass between Group M and CUMS after 14 days (P<0.05), and the body mass of 21 days was also significantly different (P<0.01). Between Group M and CUMS, in the open-field test, the upright times, crossings on Day 14 and 21, and crossings time and upright times on Day 28 were significantly reduced in Group M (P<0.01). The numbers of hippocampal neurons in Group M and CUMS were significantly less than Group Control, so were the levels of 5-HT in the hippocampus (P<0.01); but there was no significant difference between Group M and CUMS (P>0.05). Conclusion This study successfully constructes a narrow space depression rat model, with comparable evaluation indexes of classical CUMS depression rat model. Moreover, some indicators showed significant differences earlier on Day 14, indicating that M model may be able to shorten the modeling time. The modeling method is simple, stable and time-saving method.