circBPTF靶向miR-107对H2O2诱导SK-N-SH神经细胞氧化损伤的影响

Effect of circBPTF targeting miR-107 on H2O2-induced oxidative damage of SK-N-SH nerve cells

ObjectiveTo explore the effect of circBPTF onoxidative stress and apoptosis of neurons induced by hydrogen peroxide(H₂O₂)and its regulatory effect on miR-107.Methods Human neuroblastoma cells SK-N-SH were cultured in vitro, and SK-N-SH cells were induced by H₂O₂ to establish a celloxidativedamage model. si-NC, si-circBPTF, miR-NC, miR-107 mimics, si-circBPTF andanti-miR-NC, si-circBPTF and anti-miR-107 were transfected into SK-N-SH cells induced by H₂O₂. The qRT-PCR method was used to detect the expression of circBPTF and miR-107 in the cells. The kit was used to detect the release of LDH and the content of MDA and SOD. Flow cytometry was used to detect the apoptosis rate. The dual luciferase reporter experiment detects the targeting relationship between circBPTF and miR-107. Western blot was used to detect the expression of Bcl-2 and Bax protein.Results The protein expressionof circBPTF and Bax, the release of LDH, the content of MDA and apoptosis rate were significantly increased in SK-N-SH cells induced by H₂O₂ (P<0.05). On the contrary, the expression of miR-107 and the protein level of Bcl-2 was significantly decreased(P<0.05). In addition, transfection ofsi-circBPTF or miR-107 mimics could significantly reduce LDH release, MDA level, Bax protein expression and apoptotic rate of SK-N-SH cells induced by H₂O₂(P<0.05). It also significantly increased SOD content and Bcl-2 protein level(P<0.05).The dual luciferase report experiment furtherly confirmed that circBPTF could target miR-107. Co-transfection of si-circBPTF and anti-miR-107 could significantly reverse the effect of transfection of si-circBPTF on apoptosis and oxidative stress of SK-N-SH cells induced by H₂O₂.Conclusion Inhibiting the expression of circBPTF could up-regulate miR-107 and inhibit H₂O₂-induced oxidative stress and apoptosis of SK-N-SH cells, thereby reducing oxidative damage of neurons.