血浆miR-424,FGF2水平与急性缺血性脑卒中患者血管介入术后出血转化的相关性分析

目的 探讨血浆微小RNA-424(MicroRNA,miR-424)、成纤维细胞生长因子2(Fibroblast growth factor 2,FGF2)水平与急性缺血性脑卒中(Acute ischemic stroke,AIS)患者血管介入术后出血转化(Hemorrhagic transformation,HT)的...

The correlation between plasma miR-424 and FGF2 levels and hemorrhagic transformation in patients with acute ischemic stroke after vascular intervention

ObjectiveTo investigate the correlation between plasma microRNA-424(miR-424), fibroblast growth factor 2(FGF2)levels, and hemorrhagic transformation(HT)after vascular intervention in patients with acute ischemic stroke(AIS).Methods From December 2018 to March 2020, we enrolled 104 patients with AIS who underwent mechanical thrombectomy. The patients were divided into HT group(n=28)and non HT group(n=76)according to CT examination after operation. The general information and National Institutes of Health Stroke Scale(NIHSS)score at admission were collected. Plasma samples were collected before and after operation. The levels of miR-424 and FGF2 mRNA in plasma were detected by fluorescence quantitative PCR. Pearson method was used to analyze the correlation between plasma miR-424 and FGF2 levels in patients with HT. Multivariate Logistic regression was used to analyze the risk factors of HT in AIS patients. The receiver operating characteristic(ROC)curve was used to evaluate the predictive value of plasma miR-424 and FGF2 levels for HT in patients with AIS after vascular intervention.Results In the HT group, the NIHSS score at admission was higher than that of the non-HT group(P<0.05). Before operation, the expression of miR-424 was lower, while the expression of FGF2 was higher in the HT group than those in the non-HT group(P<0.05). Compared with those before operation, the plasma miR-424 in HT group and non-HT group after operation were increased, and that in the HT group was lower than that in the non-HT group. The FGF2 mRNA after operation was increased than before operation, and that in the HT group was higher than that in the non-HT group(P<0.05). Plasma miR-424 and FGF2 were negatively correlated with each other in the HT group(r=-0.629, P<0.05). Multivariate Logistic analysis showed that high NIHSS score at admission, low miR-424 level, and high FGF2 level were independent risk factors for HT in AIS patients after the vascular intervention(P<0.05). ROC results showed that the area under the curve(AUC)of preoperative plasma miR-424 and FGF2 in predicting HT in AIS patients was 0.796 and 0.820, respectively. The AUC predicted by the two methods was 0.905, and the sensitivity and specificity were 71.43% and 94.74%, respectively.Conclusion The plasma level of miR-424 in AIS patients with HT after vascular intervention is low, while the level of FGF2 is high. The predictive value for postoperative HT in AIS patients, and are helpful to evaluate the prognosis.