| 紫外分光光度法测定菟丝子提取物中总黄酮的含量 |
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| 引用本文： | 王刚,杨松松.紫外分光光度法测定菟丝子提取物中总黄酮的含量[J].辽宁中医学院学报,2009(11):188-189. |
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| 作者姓名： | 王刚 杨松松 |
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| 作者单位： | [1]遵义医学院,贵州遵义541002 [2]辽宁中医药大学,辽宁沈阳110032 |
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| 摘 要： | 目的：建立菟丝子提取物中总黄酮含量测定方法。方法：以芦丁为标样，对菟丝子提取物中黄酮类化合物采用甲醇超声提取，用紫外分光光度法，在500nm处测定吸光度。结果：总黄酮在7．456～44．736μg／mL范围内线性关系良好，其回归方程为：A=0．0123C＋0．0337（r=0．9996）；平均加样回收率为93．2％（RSD为1．3蚴。结论：该实验方法简便、快速、准确，可控制质量。
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| 关 键 词： | 菟丝子 总黄酮 紫外分光光度法 |
Quantitative Determination of Total Flavones in Cuscuta Chinensis Lain Extract by ~ Spectrophotometry |
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| WANG Gangi,YANG Song-song.Quantitative Determination of Total Flavones in Cuscuta Chinensis Lain Extract by ~ Spectrophotometry[J].Journal of Liaoning College of Traditional Chinese Medicine,2009(11):188-189. |
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| Authors: | WANG Gangi YANG Song-song |
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| Institution: | 1.Zunyi Medical College, Zunyi 563003, Guozhou, China; 2.Liaoning University of TCM, Shenyang 110032, Liaoning, China ) |
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| Abstract: | Objective ： To determnine the total flavones in Cuscuta chinensis Lain extract. Methods ： Using detection wavelength at 500rim and rutin as the standard sample,the flavones substances in Cuscuta chinensis Lam extract were determined through methyl alcohol ultrasonic extraction and ultraviolet spectrophotometer. Results ： Sample size showed a good linear relationship at the range of 7.456-44.736 μ g/mL and the curve was A=0.0123C＋0.0337（r=0.9996）.The average recovery was 93.2%and RSD was 1.3%. Conclusion：This method is easy,rapid and accurate,and can be used for quality control of Cuscuta chinensis Lam. |
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| Keywords: | Cuscuta chinensis Lain Total flavones Spectrophotom etric method |
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