lactulose和glutamine对梗阻性黄疸大鼠空肠黏膜的影响

目的:研究梗阻性黄疸时空肠黏膜的变化及lactulose和glutamine对梗阻性黄疸大鼠空肠黏膜的影响．方法:Wistar大鼠84只,随机分为4组．通过手术结扎切断大鼠胆总管得到梗阻性黄疸模型．对梗阻性黄疸大鼠分别经胃灌注lactulose和glutamine药物,比较给药前及给药后5,10 d各组大鼠空肠黏膜绒毛高度变化,同时与未行胆管结扎的假手术对照组进行比较．结果:无论胆总管结扎与否,给药前各组空肠黏膜的绒毛高度无明显差异．胆总管结扎后大鼠空肠黏膜高度减低(5 d:q=4．32,P<0．01;10 d:q=11．03,P<0．01);应用生理盐水组大鼠的空肠黏膜绒毛高度明显低于应用lactulose和glutamine组大鼠的空肠黏膜绒毛高度,且应用glutamine组与胆总管未结扎组相近(5 d:q= 3．62,P>0．05;10 d:q=3．83,P>0．05);而应用lactulose和glutamine的2组大鼠空肠黏膜绒毛高度无明显差异(P>0．05)．结论:结扎大鼠胆总管可导致其空肠黏膜萎缩．经胃肠道应用lactulose或glutamine对胆道梗阻所致的大鼠空肠黏膜萎缩均具有保护作用,且二者对肠黏膜的保护作用无明显差异．

Influence of lactulose and glutamine on the jejunum mucosa of rats with obstructive jaundice

AIM: To investigate the influence of lactulose and glutamine on the changes of jejunal mucosal morphology of rats with obstructive jaundice. METHODS: Eighty-four Wistar rats were randomly divided into 4 groups. The rats in group A served as sham operation controls, and those in group B as common bile duct ligation controls. The rats in group C and D were intragastrically treated with lactulose and glutamine, respectively, besides common bile duct ligation. The rats were sacrificed at the 0, 5th and 10th post-treatment day. The morphology of jejunum mucosa was observed and the height of jejunal villi was measured under microscope. RESULTS: The tela submucosa of jejunum had a few lymphocytes in group A, while that had more lymphocytes in group B, C and D under microscope. The height of jejunal villi in group A was not changed, and it was significantly higher than that in group B (5 d: q = 4.32, P < 0.01; 10 d: q = 11.03, P < 0.01). The height of jejunal villi in group B was obviously lower than that in group C or D, but it was not significantly different between group A and D (5 d: q = 3.62, P > 0.05; 10 d: q = 3. 83, P > 0.05). There was also no marked difference between group C and D (P > 0.05). CONCLUSION: Common bile duct ligation leads to obstructive jaundice, which results in atrophy of the jejunum mucosa. Both lactulose and glutamine may prevent the atrophy of intestinal mucosa, but their effects are not significantly different.