维吾尔药材新疆圆柏DNA 条形码鉴定研究

目的：本研究采用形态学与DNA 条形码技术，建立维吾尔药材新疆圆柏Juniperus sabina L.及其混伪品的鉴别方法。方法：随机选择不同产地新疆圆柏及其混伪品的叶片和果实，比较外观差异；采用ITS2 序列对新疆圆柏及其混伪品10 份样品进行PCR 扩增并测序，结合GenBank 获得4 条侧柏ITS2序列，比较14 份样品ITS2 序列种内和种间变异，采用K2P 模型构建NJ 系统树。结果：4 种植物叶片和果实外观相态均有相似之处；DNA 条形码ITS2 序列鉴定结果显示，14 份研究样品ITS2 序列长度均为219 bp，种间最大K2P 距离为0.089。结论：ITS2 序列及NJ 树可将新疆圆柏与3 种混伪品植物区分，为维吾尔药材新疆圆柏的标准化奠定基础。

The Identification of Uygur Medicine Juniperus sabina by DNA Barcode

To establish the DNA barcode identification method of Uygur Medicine Juniperus sabina. Leaves and fruits were selected randomly from different areas to contrast morphology; Ten samples were amplified by ITS2 sequence, combining with four ITS sequences of Platycladus orientalis obtained from GenBank, intraspecific and interspecific variation were compared, K2P and NJ methods were utilized for analysising the relationship. The results show J. sabina cannot be distinguished with its adulterants by morphological characteristics of leaves and fruits; 14 Samples of ITS2 sequence length are 219 bp, the maximum K2P distance are 0.089. J. sabina and its 3 adulterants plant can be distinguished by the DNA barcode ITS2 sequence and NJ tree successfully. Therefore, ITS2 barcodesequence was able to identify J. sabina and its adulterants species, which provides an effective way for the molecular identification of Uygur medicine J. sabina.