| LC-MS/MS法同时测定人血清中卡马西平和拉莫三嗪浓度 |
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| 引用本文： | 陆川,张梦琪,茅晓寅,贾晶莹,刘罡一,余琛. LC-MS/MS法同时测定人血清中卡马西平和拉莫三嗪浓度[J]. 中国药房, 2011, 0(6) |
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| 作者姓名： | 陆川 张梦琪 茅晓寅 贾晶莹 刘罡一 余琛 |
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| 作者单位： | 上海市徐汇区中心医院中心实验室;上海医药临床研究中心分析化学部; |
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| 摘 要： | 目的:建立同时测定人血清中卡马西平和拉莫三嗪浓度的方法。方法:采用液-质联用法,以伐地那非和红地那非为内标,血清经乙腈直接沉淀后进样分析。色谱柱为XbridgeTMPhenyl,流动相为含0.02%甲酸的水溶液-含0.02%甲酸的乙腈溶液(60∶40),流速为0.3mL·min-1。电喷雾离子源,正离子多反应监测扫描分析,卡马西平和内标伐地那非的离子对分别为m/z237→194和m/z489→72;拉莫三嗪和内标红地那非的离子对分别为m/z256→43和m/z467→111。结果:卡马西平和拉莫三嗪血药浓度分别在5～500μg·L-1(r=0.9988)和100～10000μg·L-1(r=0.9964)范围内线性关系良好,定量下限分别为5、100μg·L-1。日内、日间RSD均<8%;平均提取回收率分别在88.9%～100.9%和89.5%～100.3%范围内。结论:本方法样品处理简便,适用于临床血药浓度监测及药动学研究。
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| 关 键 词： | 卡马西平 拉莫三嗪 液-质联用法 血清 药动学 |
Simultaneous Determination of Carbamazepine and Lamotrigine in Human Serum by LC-MS/MS |
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| LU Chuan,ZHANG Meng-qi,JIA Jing-ying,LIU Gang-yi,YU Chen MAO Xiao-yin. Simultaneous Determination of Carbamazepine and Lamotrigine in Human Serum by LC-MS/MS[J]. China Pharmacy, 2011, 0(6) |
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| Authors: | LU Chuan ZHANG Meng-qi JIA Jing-ying LIU Gang-yi YU Chen MAO Xiao-yin |
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| Affiliation: | LU Chuan,ZHANG Meng-qi,JIA Jing-ying,LIU Gang-yi,YU Chen(Central Laboratory,Shanghai Xuhui District Central Hospital,Shanghai 200031,China) MAO Xiao-yin(Dept. of Analytical Chemistry,Shanghai Clinical Research Center,Shanghai 200233,China) |
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| Abstract: | OBJECTIVE: To develop the method for concentration determination of carbamazepine and lamotrigine in human serum. METHODS: With vardenafil and hongdenafil as internal standard, LC-MS was adopted to analyze serum with protein precipitated by acetonitrile. The samples were separated on XbridgeTM Phenyl (150 mm×2.1 mm,5 μm)column with mobile phase consisted of 0.02% formic acid solution-0.02% formic acid acetonitrile solution(60 ∶ 40) at flow rate of 0.3 mL·min-1. The protonated ion of samples was detected in ... |
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| Keywords: | Carbamazepine Lamotrigine LC-MS Serum Pharmacokinetic |
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