香港远志黄酮类成分对H_2O_2诱导PC12细胞损伤的保护作用研究

目的:研究香港远志黄酮类成分对H2O2损伤PC12细胞的保护作用。方法:应用现代分离鉴定技术分离香港远志中黄酮类成分;96孔板培养PC12细胞24h加入100μmol的受试样品(以维生素C为对照),培养24h后加入400μmolH2O2作用2h。使用MTT法检测各组细胞活性,用自动酶标仪在570nm波长下测定各孔吸光度。结果:香港远志中黄酮类成分山柰酚-3-O-芸香糖苷、山柰酚-3-O-葡萄糖苷、芒果苷、香港远志黄酮A、香港远志黄酮B具有一定的对H2O2损伤PC12细胞保护作用的活性。结论:本实验可为防治阿尔茨海默病新药的研发提供理论基础。

Protective Effect of Flavonoids from Polygala hongkongensis on H_2O_2 Induced PC12 Cells Damage

OBJECTIVE： To study the protective effect of flavonoids from Polygala hongkongensis on H2O2 induced PC12 cells damage. METHODS： Flavonoids of P. hongkongensis was acquired by modem isolation and identification methods; PC12 cells were cultured in 96 well plates for 24 h. Then 100 μmol of tested samples （vitamin C as control） were added. After 24 h, cells were treated with 400 μmol H2O2, for 2 h. MTT assay was used to detect activity of PC12 cell. The absorption of each well was measured by automatic reader at 570 rim. RESULTS： Kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, mangiferin, HongKong polyhongkongenoxanthone A and polyhongkongenoxanthone B from P. hongkongensis had protective effect on H2O2 induced PC12 cell damage. CONCLUSION： The study can be used as research theoretic basis of new drugs for alzheimer＇s disease.