氨基胍对人翼状胬肉成纤维细胞增殖影响的体外试验研究

目的:观察氨基胍(AG)对体外培养的人翼状胬肉成纤维细胞增殖的影响。方法:取人翼状胬肉标本进行体外贴壁细胞常规培养,传代至第3～5代细胞用于试验,以每孔1×104个细胞接种于培养板中,加入不同浓度(250、500、750、1000μmol.L-1)的AG作为AG组,另设不加AG的为对照组,检测作用24、48、72h后各孔的吸光度,并计算细胞增殖抑制率,每个时间点、每个浓度均设6个复孔。结果:人翼状胬肉成纤维细胞增殖抑制率随AG浓度的增大和作用时间的延长明显升高(P<0.05)。结论:AG在受试浓度和受试时间范围内,对体外培养的人翼状胬肉成纤维细胞具有抑制作用且呈浓度、时间依赖性。

Effect of Aminoguanidine on the Proliferation of Human Pterygium Fibroblast in Vitro

OBJECTIVE： To observe the effect of aminoguanidine （AG） on the proliferation of in vitro cultured human pterygium fibroblast. METHODS： Human pterygium specimens were obtained to conduct normal in vitro-culture of adherent cells and use the 3-5 generation cells for experiment. 1×10^4 cells were inoculated in each well of culture plate supplemented with different concentrations of AG （250, 500, 750, 1 000 μmol.L^-1） as AG group. Other culture plate weren＇t supplemented with AG as control group. The absorbency of each well were detected on 24, 48, 72 h. The inhibition rate of cell growth was detected. 6 reduplicate wells of each time point and each concentration were established. RESULTS ： With the concentration of AG increasing and acting time delay, the inhibition rate of human pterygium fibroblast growth increased significantly （P〈0.05）. CONCLUSION： In a certain range of concentration and time, AG have inhibited the proliferation of in vitro cultured human pterygium fibroblast in concentration and time-dependent manner.