钩藤HPLC指纹图谱的构建

目的:建立钩藤药材的高效液相色谱(HPLC)指纹图谱,并结合钩藤碱和异钩藤碱的含量测定结果,为钩藤药材的质量控制提供客观依据。方法:色谱柱为依利特Hypersil BDS C1(8250mm×4.6mm,5μm),流动相为甲醇-含0.02%三乙胺的水(梯度洗脱),流速为1.0mL·min-1,紫外检测波长为254nm。结果:依照10个批次样品的色谱数据构建起钩藤药材的HPLC指纹图谱,相似度评价和主成分分析的结果良好。结论:所构建的指纹图谱可以反映钩藤药材的内在组成,作为其质量评价的标准。

Establishment of HPLC Fingerprint of Ramulus Uncariae Cum Uncis

OBJECTIVE： To establish the HPLC fingerprint of Ramulus Uncariae Cure Uncis, and to provide objective basis for the quality control of Ramulus Uncariae Cum Uncis based on the results of the content determination of rhynchophylline and isorhynchophylline. METHODS： The determination was performed on Hypersil BDS C18（250 mm×4.6 ram, 5 μm） column with the mobile phase consisted of methanol-0.02% triethylamine-water （gradient elution）. The flow rate was 1.0 mL·min-1 and UV detec- tion wavelength was 254 rim. RESULTS： The HPLC fingerprints of Ramulus Uncariae Cum Uncis were established according to the chromatographic data of 10 batches of samples, The results of similarity evaluation and principle component analysis （PCA） were favorable. CONCLUSION： Established fingerprint reflects the inherent character of Ramulus Uncariae Cure Uncis and can be used for the quality evaluation of it.