| 大孔吸附树脂对红花多糖脱色工艺的影响研究 |
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| 引用本文： | 邹义芳,任爱农,姚苗苗,顾雪红.大孔吸附树脂对红花多糖脱色工艺的影响研究[J].中国药房,2011(15):1380-1382. |
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| 作者姓名： | 邹义芳 任爱农 姚苗苗 顾雪红 |
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| 作者单位： | 江苏大学药学院;江苏省中医药研究院; |
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| 摘 要： | 目的:探讨大孔吸附树脂对红花多糖脱色工艺的影响。方法:采用单因素试验,以脱色率和多糖保留率作为评价指标,比较AB-8、HPD-400A、HPD-100、HPD-750、D101、ADS-17等6种不同型号大孔吸附树脂在温度、多糖浓度、pH值、吸附流速、洗脱剂5个方面对红花多糖脱色效果的影响。结果:HPD-100大孔吸附树脂对红花多糖的脱色率和保留率较为理想。最佳工艺为40℃,多糖浓度为5 mg·mL-1,pH值为4,流速为1 ml·min-1,洗脱剂为pH 4的蒸馏水。在该条件下色素的吸附率可达86.71%,多糖保留率为72.10%。结论:HPD-100大孔树脂对红花多糖可以获得较高的脱色率和保留率。
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| 关 键 词： | 大孔吸附树脂 红花多糖 脱色 |
Effect of Macroporous Adsorptive Resins on Decoloration Technology of Carthamus tinctorius Polysaccharide |
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| ZOU Yi-fang,YAO Miao-miao,GU Xue-hong REN Ai-nong.Effect of Macroporous Adsorptive Resins on Decoloration Technology of Carthamus tinctorius Polysaccharide[J].China Pharmacy,2011(15):1380-1382. |
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| Authors: | ZOU Yi-fang YAO Miao-miao GU Xue-hong REN Ai-nong |
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| Institution: | ZOU Yi-fang,YAO Miao-miao,GU Xue-hong(School of Pharmacy,Jiangsu University,Zhenjiang 212013,China) REN Ai-nong(Jiangsu Provincial Institute of Traditional Chinese Medicine,Nanjing 210028,China) |
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| Abstract: | OBJECTIVE:To study the effect of macroporous adsorptive resin on the decoloration technology of Carthamus tinctorius polysaccharide.METHODS:The effects of 6 kinds of macroporous adsorptive resin,i.e.AB-8,HPD-400A,HPD-100,HPD-750,D101,ADS-17,on the decolorization technology of C.tinctorius polysaccharides were compared with single factor test in terms of temperature,polysaccharide concentration,pH,adsorption flow,eluant.RESULTS:The decolorization rate and polysaccharide retention rate of HPD-100 were optimal... |
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| Keywords: | Macroporous adsorptive resin Carthamus tinctorius polysaccharide Decoloration |
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