HPLC法测定复方贞术调脂胶囊中三七皂苷R_1和人参皂苷Rg_1、Rb_1的含量

目的:建立测定复方贞术调脂胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent-C1(8250 mm×4.6 mm,5μm),流动相为乙腈-水(梯度洗脱),柱温为25℃,流速为1.0 mL.min-1,检测波长为203 nm。结果:三七皂苷R1、人参皂苷Rg1与人参皂苷Rb1的进样量分别在0.206 4～6.192 0、0.370 6～11.118 0、0.355 0～10.650 0μg之间与各自峰面积积分值呈良好线性关系(r均为0.999 9);平均回收率分别为97.83%、97.08%和96.92%,RSD分别为0.92%、0.27%和0.36%(n均为6)。结论:本方法简便、准确、重复性好,可用于复方贞术调脂胶囊的质量评价。

Content Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Ginsenoside Rb_1 in Compound Zhenshu Tiaozhi Capsules by HPLC

OBJECTIVE：To establish a method for the content determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Compound zhenshu tiaozhi capsules.METHODS：HPLC method was adopted.The chromatographic column was Agilent C18（250 mm×4.6 mm,5 μm） column.The mobile phase was consisted of acetonitrile-water at flow rate of 1.0 mL·min-1（gradient elution）.The detection wavelength was set at 203 nm and the column temperature was 25 ℃.RESULTS：There was good linearity in the range of 0.206 4～6.192 0 μg for notoginsenoside R1,0.370 6～11.118 0 μg for ginsenoside Rg1 and 0.355 0～10.650 0 μg for ginsenoside Rb1.Their average recoveries were 97.83%,97.08% and 96.92%,respectively,and the corresponding RSD were 0.92%,0.27% and 0.36%,respectively.CONCLUSION：The method is simple,accurate and reproducible,and applicable for the quality control of Compound zhenshu tiaozhi capsules.