印楝叶HPLC指纹图谱及其化学成分与遗传变异的关系研究

目的:建立印楝叶的高效液相色谱(HPLC)指纹图谱,并分析其化学成分与遗传变异的关系。方法:采用HPLC法进行指纹图谱研究,运用简单序列重复间扩增多态性(ISSR)技术分析遗传多样性,利用聚类分析研究二者的关系。结果:建立了印楝叶药材的HPLC指纹图谱,共标定了11个共有指纹峰。分别根据Nei’s遗传距离和化学成分进行聚类分析,13个种源分为两大类:来自缅甸的11个种源聚成一类;来自澳大利亚和印度的种源聚成另外一类。结论:所建立的指纹图谱稳定、可靠、重复性好;不同种源印楝叶药材化学成分与遗传变异之间具有一定的相关性。

Study on the HPLC Fingerprint of the Leaf of Azdirachta indica and Relationship of Its Chemical Constituents with Genetic Variation

OBJECTIVE： To establish the HPLC fingerprint of the Azadirachta indica leaf and to analyze the relationship between its chemical constituents and genetic variation. METHODS ： HPLC fingerprint was studied. ISSR technique was used to analyze the genetic diversity. The relationship between chemical constituents and genetic variation was analyzed by cluster analysis. RESULTS： The HPLC fingerprint of the leaf ofA. idica was established, there were 11 common peaks. According to Nei＇ s genetic distances and chemical constituents, cluster analysis was conducted. 13 provenances were divided into 2 clusters： one was consisted of 11 provenances of Burma, and the other was Australia and India provenances. CONCLUSION： Established HPLC fingerprint is stable and reliable with good reproducibility. There are certain correlations between the genetic variation and chemical constituents.