| 无花果叶补骨脂素对体外培养瘢痕疙瘩成纤维细胞的影响 |
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| 引用本文： | 王涛,陶如,李清华,高永峰,周成军,刘金成. 无花果叶补骨脂素对体外培养瘢痕疙瘩成纤维细胞的影响[J]. 中国药房, 2011, 0(19): 1746-1749 |
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| 作者姓名： | 王涛 陶如 李清华 高永峰 周成军 刘金成 |
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| 作者单位： | 泰山医学院基础医学部生物化学教研室;泰山医学院临床医学部;泰山医学院附属医院;泰山医学院药学院药学综合实验室;山东大学第二医院;青岛大学医学院生物学教研室; |
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| 基金项目： | 山东省中医药管理局资助项目(2007167); 泰山医学院青年基金资助项目(997) |
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| 摘 要： | 目的:研究无花果叶补骨脂素(FLP)对瘢痕疙瘩成纤维细胞的生长抑制作用。方法:用FLP处理体外培养的成纤维细胞后进行细胞形态学检测,通过细胞增殖试验(MTT法)和流式细胞仪检测FLP对成纤维细胞增殖和凋亡的影响;氯胺T法检测FLP对成纤维细胞胶原蛋白合成的抑制作用;Western-bloting检测凋亡相关蛋白Bcl-2的表达。结果:经FLP处理后,成纤维细胞增殖活性降低,凋亡指数升高(P<0.01),凋亡相关蛋白Bcl-2表达被抑制,同时胶原蛋白的合成受到抑制。结论:FLP对瘢痕疙瘩成纤维细胞增殖的抑制和诱导其凋亡作用可能是通过抑制凋亡相关蛋白Bcl-2的作用来实现的。
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| 关 键 词： | 无花果叶 补骨脂素 瘢痕疙瘩 成纤维细胞 增殖 凋亡 |
Influence of Psoralen of Fig Leaf on Human Keloid Fibroblasts in Vitro |
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| WANG Tao TAO Ru LI Qing-hua GAO Yong-feng ZHOU Cheng-jun LIU Jin-cheng(Dept. of Biology,Medical College of Qingdao University,Qing.... Influence of Psoralen of Fig Leaf on Human Keloid Fibroblasts in Vitro[J]. China Pharmacy, 2011, 0(19): 1746-1749 |
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| Authors: | WANG Tao TAO Ru LI Qing-hua GAO Yong-feng ZHOU Cheng-jun LIU Jin-cheng(Dept. of Biology Medical College of Qingdao University Qing... |
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| Affiliation: | WANG Tao(Dept. of Biochemistry,Basic Medical College,Taishan Medical University,Taian 271000,China) TAO Ru(Medical College,China) LI Qing-hua(The Affiliated Hospital of Taishan Medical University,China) GAO Yong-feng(General Lab of Pharmacy,College of Pharmacy,China) ZHOU Cheng-jun(The Second Hospital of Shandong University,Jinan 250033,China) LIU Jin-cheng(Dept. of Biology,Medical College of Qingdao University,Qing... |
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| Abstract: | OBJECTIVE:To investigate the effect of psoralen of fig leaf(FLP) on the proliferation and apoptosis of human keloid fibroblasts. METHODS:Human keloid fibroblasts were treated with FLP. Morphology,MTT method and flow cytometry(FCM) were adopted to detect the effect of FLP on the proliferation and apoptosis of human keloid fibroblasts. The inhibition effect of PSO on the synthesis of fibroblasts collagen was determined by chloramines T assay. The protein levels of Bcl-2 were detected by Western bloting. RESUL... |
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| Keywords: | Fig leaf Psoralen Human keloid Fibroblasts Proliferation Apoptosis |
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