2种测定雄黄和牛黄解毒片中可溶性砷含量的方法比较

目的:建立和完善中药中可溶性砷含量测定的方法。方法:采用稀盐酸和人工胃液2种溶剂分别提取雄黄和牛黄解毒片中的可溶性砷,比较古蔡氏法和二乙基二硫代氨基甲酸银(DDC-Ag)法测定可溶性砷含量的结果。结果:古蔡氏法测定样品中的可溶性砷符合2005年版《中国药典》(一部)的要求;DDC-Ag法测定可溶性砷浓度在0～6.00μg·mL-1范围内与吸光度呈良好的线性关系(r=0.9993),平均回收率为99.73%,RSD=2.88%。结论:2种方法各有优缺点。古蔡氏法测中药中可溶性砷含量,方法简单,成本低;但DDC-Ag法比较准确,成本较低,两法均可作为中药中微量可溶性砷含量的测定方法。

Comparison of 2 Kinds of Determination Methods of Soluble Arsenic in Realgar and Niuhuang Jiedu Tablets

OBJECTIVE： To perfect the determination method of arsenic in traditional Chinese medicine. METHODS： Diluted hydrochloric acid and artificial gastric fluid were used to extract arsenic from realgar and Niuhuang jiedu tablets. The content of arse- nic in TCM was determined by original Chua＇s method and DDC-Ag method, compare 2 kinds of methods for the content determi- nation of soluble arsenic in realgar and Niuhuang jiedu tablets. RESULTS： Determination of arsenic by original Chua＇ s method meet the 2005 edition of ＂Chinese pharmacopceia＂（a） requirements. The linear range of arsenic was 0-6.00 μg·mL-1 （r=0.999 3） with an average recovery of 99.73% （RSD=2.88%）. CONCLUSION： Original Chua＇ s method is simple with low cost. DDC-Ag method is more accurate, lower cost than the former and it can be used for the content determination of soluble arsenic in TCM.