| RP-HPLC法测定人血浆中拉莫三嗪的浓度 |
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| 引用本文： | 王来成,王涛,韩强,吕冬梅,朱虹.RP-HPLC法测定人血浆中拉莫三嗪的浓度[J].中国药房,2011(10):887-889. |
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| 作者姓名： | 王来成 王涛 韩强 吕冬梅 朱虹 |
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| 作者单位： | 徐州医学院附属医院药剂科;徐州医学院药学院; |
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| 摘 要： | 目的:建立测定人血浆中抗癫痫药拉莫三嗪的方法。方法:采用反相高效液相色谱法,以萘普生为内标,血浆样品以直接检蛋白测沉波淀长法为处28理0n。m色。谱结柱果为:拉X莫Te三rra嗪?R血P药18浓,流度动在相0.为5～甲3醇2.0-0m.0g1.mL-o1l.范L围-1磷内酸线二性关氢系钾良缓好冲(液r(=pH0.969.09)(9)6,6最:3低4)检,流测速浓为度为1.00m.2Lm.gm.iLn--11,,提取回收率为68.20%～82.35%,方法回收率为99.03%～103.21%,日内、日间RSD均<4.00%。结论:本方法操作简便,灵敏度高,重复性好,能快速准确测定人血浆拉莫三嗪浓度。
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| 关 键 词： | 拉莫三嗪 反相高效液相色谱法 血药浓度 |
Determination of Lamotrigine in Human Plasma by RP-HPLC |
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| WANG Lai-cheng,WANG Tao,HAN Qiang,LV Dong-mei ZHU Hong.Determination of Lamotrigine in Human Plasma by RP-HPLC[J].China Pharmacy,2011(10):887-889. |
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| Authors: | WANG Lai-cheng WANG Tao HAN Qiang LV Dong-mei ZHU Hong |
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| Institution: | WANG Lai-cheng,WANG Tao,HAN Qiang,LV Dong-mei(Dept.of Pharmacy,The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,China) ZHU Hong(School of Pharmacy,Xuzhou Medical College,China) |
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| Abstract: | OBJECTIVE： To develop the method for the determination of lamotrigine in human plasma. METHODS： RP-HPLC was adopted. The samples were extracted by direct protein precipitation using naproxen as internal standard. XTerra RP18 column was used with the mobile phase consisted of methanol-0.01 mol .L^-1 potassium dihydrogen phosphate buffer（pH 6.0）（66：34）. The flow rate was 1.0 mL. min-~, and the detection wavelength was set at 280 nm. RESULTS： The linear range of lamotrigine was 0.5~32.0 mg-L-t（r=0.999 9）. The lowest detection concentration was 0.2 mg-L-~. The extraction recovery was 68.20%~82.35% and the method recovery was 99.03% ~103.21%. The RSD of intra-day and inter-day were both below 4.00%. CONCLUSION： The method is simple, sensitive and reproducible for concentration determination of lamotrigine in human plasma |
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| Keywords: | Lamotrigine RP-HPLC Plasma concentration |
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