RP-HPLC法测定赤芍总苷原料中芍药苷和赤芍总苷的含量

目的:建立测定赤芍总苷原料中芍药苷和赤芍总苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为YMC-PackODS-A(250mm×4.6mm,5μm),流动相为乙腈-0.02mo·lL-1磷酸二氢钠(20∶80),检测波长分别为230、227nm。结果:芍药苷与赤芍总苷(以苯甲酸计)的进样量分别在0.832～4.160μg(r=0.9993)、0.0224～0.1120μg(r=0.9991)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为104.2%、105.2%,RSD分别为1.96%、1.94%(n均为9)。结论:本方法简便、易行,精密度、重复性、稳定性好,适用于赤芍总苷原料的分析测定。

Content Determination of Peoniflorin and Total Paeony Glycoside in Paeonia Materials by RP-HPLC

OBJECTIVE： To establish determination methods of peoniflorin and total paeony glycoside in paeonia materials. METHODS： The content of peoniflorin and total paeony glycoside were determined by RP-HPLC. The determination was performed on YMC-Pack ODS-A （250 mm×4.6 mm, 5μm） with mobile phase consisted of acetonitrile-0.02 mol·L^-1 NaH2PO4（20： 80） at detection wavelength of 230nm and 227 nm. RESULTS： The linear range of peoniflorin and total paeony glycoside were 0.832-4.160 μg（r=0.999 3）and 0.022 4-0.112 0 p.g （r=0.999 1）, respectively. The average recoveries were 104.2% and 105.2% respectively. RSD were 1.96% and 1.94% （n=9）. CONCLUSION： The method is simple and easy for determination and analysis of paeonia materials. The method is characterized with the features of accuracy, reproducibility and stability.