外源性GM1对癫痫大鼠脑损伤的保护作用

目的　探讨外源性神经节苷脂GM1对癫痫大鼠脑损伤有无保护作用。方法　采用硫代氨基脲 (7.5mg/kg)诱导大鼠癫痫发作模型 ,用免疫组化方法动态观察致痫组大鼠和GM 1干预组大鼠在癫痫发作 2 4小时、4 8小时、72小时及 7天时 ,以及正常对照组、生理盐水组大鼠 72小时时神经生长因子 (NGF)在实验大鼠海马及额叶神经细胞表达情况。同时应用电镜技术观察受损海马神经细胞形态及结构变化。结果　正常对照组和生理盐水组大鼠无癫痫发作 ,致痫组和GM1干预组大鼠有Ⅰ Ⅴ级癫痫发作。免疫组化结果显示 ,癫痫鼠在其海马、额叶有较多的NGF阳性神经细胞表达 ,而未致痫鼠偶有NGF阳性细胞表达 (P <0 .0 5 )。在致痫鼠中 ,GM1干预后NGF表达明显高于未干预组 (P <0 .0 5 ) ,且以 72小时NGF表达为最高(P <0 .0 5 )。电镜显示癫痫鼠神经细胞损伤 ,但GM1干预后损伤减轻 ,而正常对照组和生理盐水组神经细胞形态和结构正常。结论　癫痫发作可引起脑细胞损伤 ;GM1对癫痫大鼠脑损伤具有一定保护作用 ,其保护作用可能通过诱导NGF表达增多来实现。

The protective effect of exogenous GM1 on injured cerebral nerve cells of epileptic wistar rats

Objective To study the protective effect of exogenous GM1 (monosialoganglioside) on injured cerebral nerve cells of epileptic wistar rats induced by thiosemicarbazied.Methods 41 wistar rats were divided into four groups: group A (normal control group), group B (the rats treated with NS), group C (epileptic rats with thiosemicarbazied) and group D (epileptic rats treated with GM1). A method of immunohistochemistry was used to study the expression of NGF (Nerve growth factor) on the hippocampal and frantal lobe nerve cells of epileptic rats from groups C and D at 24 hours, 48 hours, 72 hours and 7 days respectively. Some rats from groups A and B at 72 hours were also studied by this method. At the same time, the shape and construction of cerebral nerve cells from four groups at 72 hours were observed by electromicroscope. Results The number of NGF positive cells was significant in the epileptic groups (groups C and D) as compared with non epileptic grous (groups A and B) ( P < 0.05 ). Further more, NGF expression of group D was remarkably increased as compared with group C. The study of pathology showed that the nerve cells of groups A and B were intact and the nerve cells of groups C and D weren't. After treated with exogenous GM1, the injured nerve cells were improved greatly. Conclusions Seizures can injure the hippocampal and frantal lobe nerve cells. Exogenous GM1 can protect the injured nerve cells after seizures and it can exert the protective function by inducing the increase of NGF expressions.