| 人参瓜蒌莪术汤对小鼠lewis肺癌细胞增殖的影响 |
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| 引用本文： | 倪娅,邱幸凡. 人参瓜蒌莪术汤对小鼠lewis肺癌细胞增殖的影响[J]. 湖北中医药大学学报, 2009, 11(4): 8-10 |
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| 作者姓名： | 倪娅 邱幸凡 |
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| 作者单位： | 湖北中医学院基础医学院,湖北武汉,430065;湖北中医学院基础医学院,湖北武汉,430065 |
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| 摘 要： | 目的 探讨人参瓜萎莪术汤对lewis肺癌模型小鼠的治疗作用及机制.方法 建立小鼠Lewis肺癌移植瘤模型,分为模型对照组、环磷酰胺对照组和人参瓜萎莪术汤高、低剂量组.在计算抑瘤率的基础上,运用酶联免疫吸附剂测定法(ELISA)检测瘤组织细胞核抗原(PcNA)和细胞周期蛋白D1(Cyclin D1)的表达.结果 人参瓜萎莪术汤对小鼠Lewis肺癌表现出明显的抑制作用,以中剂量组效果最为明显,抑瘤率可达47.88%;人参瓜萎莪术汤低、中、高剂量组均能明显降低瘤组织细胞PCNA的表达(P＜0.01),中、高剂量组的抑制作用显著强于低剂量组(P＜0.01);同时,人参瓜萎莪术汤低、中、高剂量组均能明显降低瘤组织细胞CyclinD1的表达(P＜0.05),中剂量组效果最为显著,并明显强于环磷酰胺阳性对照组(P＜0.01).结论 人参瓜萎莪术汤具有抑制小鼠Lewis肺癌生长的作用,其作用机制可能与降低肿瘤组织Cyclin D1的表达,抑制瘤细胞的增殖有关.
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| 关 键 词： | 人参瓜萎莪术汤 肺癌 补肺通络解毒法 细胞周期蛋白D1 细胞核抗原 |
Effect of Renshen Gualou Ezhu Decoction on Proliferation of Mouse Lewis Lung Cancer Cells |
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| NI Ya,QIU Xin-fan,. Effect of Renshen Gualou Ezhu Decoction on Proliferation of Mouse Lewis Lung Cancer Cells[J]. Journal of Hubei University of Chinese Medicine, 2009, 11(4): 8-10 |
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| Authors: | NI Ya QIU Xin-fan |
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| Affiliation: | Hubei University of Chinese Medicine;Hubei Wuhan;430061;China |
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| Abstract: | Objective To explore the influence of treatment and mechanism by Renshen Gualou Ezhu Decoction(RGE) to mouse Lewis lung cancer cells.Methods Based on establishment of international standard model for tumor study-Lewis lung cancer in C57BL/6 Mice model,they calculated the inhibition rate and used ELISA to detect the PCNA and Cyclin D1.Fifty C57BL/6 Mice were divided into 5 groups: control group,low-dose RGE group,middle-dose RGE group,high-dose RGE group and Cyclophosphamide(CP) control group.Results Inhibit... |
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| Keywords: | Renshen Gualou Ezhu Decoction lung cancer Cyclin D1 Method of tonifying lung smoothing collaterals and detoxification PCNA |
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