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| 表达盒两侧不同核基质结合区序列介导表达载体的构建 | |
| 引用本文： | 王芳,孙爱平,焦云娟,王天云.表达盒两侧不同核基质结合区序列介导表达载体的构建[J].新乡医学院学报,2009,26(1):8-10. |
| 作者姓名： | 王芳 孙爱平 焦云娟 王天云 |
| 作者单位： | 1. 新乡医学院生物化学与分子生物学教研室,河南,新乡,453003 2. 新乡医学院免疫学教研室,河南,新乡,453003 3. 新乡医学院病理学教研室,河南,新乡,453003 |
| 基金项目： | 河南省科技厅自然科学基金 |
| 摘 要： | 目的构建表达盒两侧含有不同来源核基质结合区(MAR)序列的载体pCCF-βg,为进一步研究MAR的调控功能提供基础。方法用限制酶酶切质粒pCCM-in,将切下的interferon-MAR片段连接至表达盒一侧含有globin-MAR的表达载体上,构建表达盒两侧含异源MAR的表达载体pCCF-βg。结果酶切及琼脂糖凝胶电泳证实所构建的载体pCCF-βg正确,pCCF-βg表达盒两侧分别含有interferon-MAR和globin-MAR片段。结论成功构建了表达盒两侧含有不同来源的MAR片段的载体pCCF-βg。 |
| 关 键 词： | 核基质结合区 转基因 表达载体 |
Construction of the expression vector which contains two different matrix attachment regions flanking the expression cassette |
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| WANG Fang,SUN Ai-ping,JIAO Yun-juan,et al.Construction of the expression vector which contains two different matrix attachment regions flanking the expression cassette[J].Journal of Xinxiang Medical College,2009,26(1):8-10. | |
| Authors: | WANG Fang SUN Ai-ping JIAO Yun-juan |
| Institution: | WANG Fang1,SUN Ai-ping2,JIAO Yun-juan3,et al |
| Abstract: | Objective To construct the expression vector pCCF-βg which contains two different matrix attachment regions （MARs） flanking the expression cassette,in order to study the regulation function of MAR further. Methods The vector pCCM-in was cut by restriction enzymes,interferon-MAR was recovered and ligated into the vector pCCM3-βg which contains globin-MAR, the pCCF-βg vector which contains two different MARs flanking the expression cassette was constructed. Results The pCCF-βg vector which contains globin-MAR and interferon-MAR flanking the expression cassette was proved to be right through digestion with the corresponding enzymes and agarose gel electrophoresis. Conclusion The expression vector pCCF-βg which contains the different MARs flanking the expression cassette was successfully constructed. |
| Keywords: | matrix attachment region transgene expression vector |
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