良性前列腺增生、前列腺癌组织内皮素-1的表达及其意义

目的:探讨内皮素1(ET-1)在BPH及PCa中的表达及其临床意义。方法:应用免疫组化ElivisionTM plus法对36例BPH和44例PCa组织中ET-1分别进行免疫组化染色,并在光镜下判断染色部位和染色强度。结果:ET-1在BPH和PCa组织中阳性表达率为100%;阳性染色部位主要位于肿瘤和增生的腺上皮的胞质,以及间质平滑肌细胞胞质;间质血管内皮细胞染色均为阳性;比较BPH和PCa的ET-1表达强度,两者差异无显著性(P>0.05);低分化PCa的ET-1表达强度显著高于高、中分化PCa(P<0.01);在骨转移(BM)PCa和非骨转移(non-BM)癌之间,ET-1表达强度差异无显著性(P>0.05)。结论:ET-1在BPH和PCa组织中均有高比例的表达,且两者的表达定位相同。ET-1与BPH和PCa的发生发展均有一定的相关性。

Expressions of Endothelin-1 in Benign Prostatic Hyperplasia and Prostate Cancer and Their Significance

Objective:To investigate the different expressions of endothelin-1(ET-1)in prostate cancer(PCa)and benign prostatic hyperplasia(BPH)tissues and their clinical significance.Methods:A total of 36 BPH and 44 PCa specimens were examined for the expression of ET-1 by immunohistochemical technique(ElivisionTM plus method).The staining intensity for ET-1 was assessed by light microscopy on a scale from "-" to "+++".Results:Positive immunoreactivity was found in BPH and PCa,with a positive rate of 100%.Positive staining was located mostly in the cytoplasm of glandular epithelia and smooth muscle cells of both BPH and PCa and was noted in all stroma vascular endothelial cells.There were no significant differences in the intensity of positive staining for ET-1 between the groups of BPH and PCa(P>0.05),bone metastasis(BM)and non-BM(P>0.05),and highly and moderately differentiated PCa(P>0.05),but the staining intensity for ET-1 was significantly higher in the poorly than in the highly and moderately differentiated PCa(P<0.01).Conclusion:ET-1 has a high expression and the localization is the same in both BPH and PCa.It is involved in the development and progression of BPH and PCa.