HPLC法测定复方齐多夫定片中齐多夫定和拉米夫定的含量

目的建立HPLC法测定复方齐多夫定片中齐多夫定和拉米夫定的含量。方法色谱柱为In-ertsil C18柱(4.6 mm×250 mm,5μm),流动相为磷酸盐缓冲液-甲醇(体积比77∶23),流速为1.0 mL.min-1,检测波长为270 nm。结果齐多夫定的线性范围为20~300 mg.L-1,r=0.999 9,平均回收率为99.4%,RSD=0.24%;拉米夫定的线性范围为10~150 mg.L-1,r=0.999 9,平均回收率为99.8%,RSD=0.33%。结论本方法快速、准确,分离效果好,适用于该复方制剂中2种主成分的同时测定。

Determination of the contents of zidovudine and lamivudine in compound zidovudine tablets by HPLC

Objective To establish an HPLC method for the determination of the content of zidovudine and lamivudine in compound zidovudine tablets. Methods A Inertsil C₁₈ column（4.6 mm× 250 mm，5 μm） was used with phosphate buffer solution and methanol(V:V=77:23) as the mobile phase, with flow rate of 1.0 mL·min^-1 and the detection wavelength of 270 nm. Results The linear range of zidovudine was 20-300 mg·L^-1，r = 0.999 9, the average recovery was 99.4% and the RSD was 0.24%; the linear range of lamivudine was 10-150 mg·L^-1，r = 0.999 9, the average recovery was 99.8% and the RSD was 0.33%. Conclusion The method is convenient, sensitive and accurate for the determination of two components in the drug.