Plasmid-dependent co-mutation in Streptomyces coelicolor A3(2) |
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Authors: | Anna Maria Puglia Fara Misuracal Rosalba Randazzo Giulia Sciandrellol Giuseppe Sermonti |
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Institution: | (1) Istituto di Genetica, Università, via Arch irafi 22, 90123 Palermo, Italy;(2) Istituto di Biologia Cellulare, Università, via Elce di Sotto, 06100 Perugia, Italy |
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Abstract: | Summary A spontaneous chromosomalmutation(plc A–) in Streptomyces coelicolor A3(2) made the inborn plasmid SCP1 susceptible to curing by UV irradiation. Lack of the SCP1 plasmid (in SCP1– strains) prevented the occurrence of the co-mutation process after MNNG treatment, although it left the susceptibility to the lethal effect of the mutagen virtually unaffected. SCP1– strains were, however, ultrasensitive to the lethal effect of UV. Curing a plc A– strain of its SCPI plasmid made it refractory to co-mutation by MNNG and sensitive to the lethal effect of UV; reinfected by the plasmid, the strain resumed both the co-mutation proficiency and the UV-resistance. The occurrence on the SCP1 plasmid of a gene comparable to the uvrE gene of E. coli (Nevers and Spatz 1975) was assumed. |
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Keywords: | Plasmid Streptomyces coelicolor Co-mutation Nitrosoguanidine |
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