清宫胶囊对药物流产后大鼠血清E2 及子宫NO,NOS水平的影响

目的:观察清宫胶囊对药物流产后大鼠血清E2及子宫组织匀浆一氧化氮(NO)含量和一氧化氮合酶(NOS)活性的影响,探讨其疗效机制。方法:将受孕的雌性大鼠50只随机分成正常妊娠组、模型对照组、益母草颗粒冲剂组、清宫胶囊低、高剂量组,采用米非司酮和米索前列醇造成流产模型后,分别ig生理盐水、益母草颗粒混悬液(14 g.kg-1.d-1),清宫胶囊混悬液(1.9,3.8 g.kg-1.d-1)7 d,检测各组大鼠血清雌二醇(E2)含量及子宫匀浆NO含量和NOS活性,观察清宫胶囊对药物流产后子宫出血的作用。结果:清宫胶囊组血清E2含量明显高于模型组,NO,NOS明显低于模型组。结论:清宫胶囊治疗药物流产后出血的作用机制可能与升高大鼠血清中E2水平,降低NO,NOS水平有关。

Effect of Qingong Capsule on Estrogen in Blood, Content of Nitric Oxide and Activity of Nitric Oxide Synthase in Uterus Homogenate of Rats after Medicine Induced Abortion

Objective: To explore the mechanism of Qingong capsule on uterine hemorrhage after medicine induced abortion. Method : Induced abortion models were made with pregnant rats drenched with mifeprisrone and misoprostol. The level of estrogen in blood, contents of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) in uterus homogenate of the rats after medicine induced abortion in every group were measured and compared. The action of Qingong (1.9, 3.8 g ·kg^-1 ·d^-1) capsule on medicine induced abortion was observed. Result : The Estrogen content in uterus homogenate Qingong capsule group was obviously higher than that in model group, and the content of NO and NOS were obviously lower than those in mdoel group. Conclusion : The mechanism of Qingong capsule on uterine haemorrhagia after medicine induced abortion may be related with improving the level of estrogen in blood and reducing the contents of NO and NOS in uterus tissue, thus it has better preventive and therapeutic effects on vaginal bleeding after medical abortion.