经前舒颗粒对经前期综合征肝气郁证 大鼠海马和下丘脑5-羟色胺转运体表达的影响

目的:研究经前舒颗粒对经前期综合征(PMS)肝气郁证大鼠海马、下丘脑5-羟色胺转运体(5-HTT)表达的影响,从分子水平探讨经前舒颗粒的作用机制。方法:雌性SD大鼠随机分为正常对照组、肝郁模型组、经前舒颗粒给药组(10 g.kg-1)和氟西汀给药组(0.002 5 g.kg-1),束缚造模法复制PMS肝气郁证大鼠模型,给药组大鼠ig给药5 d后,旷场实验、糖水偏好实验对模型进行评价,采用逆转录聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测大鼠海马和下丘脑5-HTT的mRNA及蛋白的表达。结果:与肝郁模型组相比经前舒和氟西汀给药组大鼠糖水偏好指数及旷场实验得分均显著升高(P<0.05,P<0.01);经前舒和氟西汀给药均能显著上调海马和下丘脑中5-HTT的mRNA和蛋白的表达(P<0.05,P<0.01),两给药组间无显著性差异。结论:经前舒颗粒的作用机制可能是通过调节海马、下丘脑中5-HTT的表达而发挥作用。

Effects of Jingqianshu Granule on Expression of 5-Serotonin Transpoter of Premenstrual Syndrome Model Rats with Liver-qi Stagnation

Objective: To research the effects of Jingqianshu granule (JQSG) on the expression of serotonin transporter(5-HTT)of premenstrual syndrome (PMS) model rats with liver-qi stagnation in different brain regions and the mechanism at molecular level. Method : SPF female SD rats were randomly divided into normal control group, model group, JQSG group(10 g ·kg^-1)and fluoxetine group(0.002 5 g ·kg^-1).The PMS model rats with liver-qi stagnation were induced by bondaging the limbs. Drugs were given for 5 days, and the effects were evaluated by open-field test and sucrose preference test. We analyzed the expression of 5-HTT in hippocampus and hypothalamus by the method of RT-PCR and Western blot. Result : Compared with the model group, the score of open-field and saccharine preference index of JQSG and fluoxetine group were markedly increased (P<0.05, P<0.01). The treatment could improve the expression of 5-HTT in hippocampus and hypothalamus (P<0.05,P<0.01)and the two treated groups showed no significant difference. Conclusion : JQSG can regulate the 5-HTT expression level in hippocampus and hypothalamus in model rats, which may be one of the mechanisms responsible for treating PMS with liver-qi stagnation.