| 和厚朴酚对结肠癌细胞生长影响及Caspase凋亡途径相关蛋白的表达研究 |
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| 引用本文： | 谭茵,莫立乾,蔡玉婷,张超. 和厚朴酚对结肠癌细胞生长影响及Caspase凋亡途径相关蛋白的表达研究[J]. 实用医学杂志, 2011, 27(14). DOI: 10.3969/j.issn.1006-5725.2011.14.008 |
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| 作者姓名： | 谭茵 莫立乾 蔡玉婷 张超 |
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| 作者单位： | 1. 广州医学院基础学院,510182
2. 广州医学院药学系,510182 |
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| 基金项目： | 广州市高校科技计划基金资助 |
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| 摘 要： | 目的:探讨和厚朴酚对人结肠癌(SW480)细胞增殖抑制作用及诱导Caspase凋亡途径的研究.方法:利用MTT检测和厚朴酚在不同浓度和时问下对SW480细胞体外增殖的影响;用流式细胞术、Hoechst33258荧光染色检测及Caspase-3,-8,-9 酶活性检测方法和厚朴酚诱导下结肠癌SW480细胞凋亡指标及路径.结果:MTT结果显示和厚朴酚具明显抑制SW480细胞的体外增殖,其抑制率存在剂量和时间依赖性;药物处理24,48,72 h的IC50值分别是54.36、44.72、36.20 μmol/L.和厚朴酚作用细胞后,G0/G1期的细胞比例随药物浓度增加而逐渐增多(P<0.05),表明和厚朴酚能阻滞细胞于G0/G1期.荧光染料 Hoechst33258染色结果显示.细胞核出现典型的凋亡小体.Caspase家族蛋白酶活性检测结果,胞内Caspase-3,-8,-9酶家族分别被激活.其活性随不同时间点升高(P<0.05).结论:和厚朴酚能明显抑制人结肠癌SW480细胞体外增殖,并诱导大肠癌细胞SW480通过激活Caspase途径发生凋亡.
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| 关 键 词： | 和厚朴酚 SW480细胞 凋亡 Caspase家族 |
Effects of Honokiol on the Proliferation and the Apoptosis Related Protein Expression of Caspase pathway in Human Colorectal Carcinoma Cell Line SW480 in vitro |
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| TAN Yin,MO Li-qian,CAI Yu-ting,ZHANG Chao. Effects of Honokiol on the Proliferation and the Apoptosis Related Protein Expression of Caspase pathway in Human Colorectal Carcinoma Cell Line SW480 in vitro[J]. The Journal of Practical Medicine, 2011, 27(14). DOI: 10.3969/j.issn.1006-5725.2011.14.008 |
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| Authors: | TAN Yin MO Li-qian CAI Yu-ting ZHANG Chao |
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| Affiliation: | TAN Yin*,MO Li-qian,CAI Yu-ting,ZHANG Chao.*School of Basic Medical,Guangzhou Medical College Guangzhou 510182,China |
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| Abstract: | Objective To assess the inhibitory effects of Honokiol on proliferation and inducing effect on caspase pathway in human colorectal carcinoma cell line SW480 in vitro. Methods Cultured SW480 cells were treated with Honokiol Under different concentration and time(24, 48, 72, 96 h). Cell proliferation was assessed by MTT colorimetric assay. Cell apoptosis was determined by flow cytometry (FCM) and Hoechst 33258 fluorescent staining. Caspase-3,-8,-9 activity were measured at different time points after Honokiol... |
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| Keywords: | Honokiol SW480 Apoptosis Caspase |
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