Obstructive effects of ultrasonic microbubble intensifier on CHG-5 cell with survivin antisense oligonucleotides transfection |
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Authors: | Hong-ying Cao You-de Cao Zhi-gang Wang Pan Li |
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Institution: | [1]Department of Pathology, Chongqing Medical University, Chongqing 400016 [2]Institute of Ultrasound Image, Chongqing Medical University, Chongqing 400010 |
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Abstract: | Objective:To study the effects on human glioma cell line CHG-5 by ultrasonic microbubble intensifier with survivin antisense oligonucleotides (ASODN)transfection. Methods: Antisense oligonucleotides targeting survivin mRNA was designed and synthesized.Four regimen groups were designed,group A:survivin antisense oligonucleotides transfected with ultrasonic microbubble intensifier combined with ultrasound irradiation,group B: survivin antisense oligonucleotides transfected with lipofectamine combined with ultrasound irradiation,group C:survivin antisense oligonucelotides with lipofectamine transfection.group D:blank control.The expression changes of surviving protein were measured by immunohistochemical staining and Western blotting,and MTr assay was used to measure the changes of proliferation.Results:Survivin protein expression in group A was decreased significantly in human glioma cell line CHG-5 than other groups(P<0.05),and the proliferating rate of CHG-5 in group A was also significantly inhibited(P<0.05).Conclusion:Ultrasonic microbubble intensifier transfection combined with ultrasound irradiation is a promising method in gene transfection effectively and noninvasively. |
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Keywords: | Survivin gene Antisense oligonucleotide Intensifier Ultrasonic microbubble Cell transfection |
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