| 兔角膜内皮细胞的新法培养 |
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| 引用本文： | 杨鹏,刘瑶,曾水林,张会保. 兔角膜内皮细胞的新法培养[J]. 东南大学学报(医学版), 2001, 20(3): 170-172 |
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| 作者姓名： | 杨鹏 刘瑶 曾水林 张会保 |
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| 作者单位： | 1. 东南大学医学院人体解剖学教研室
2. 东南大学医学院眼科学教研室 |
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| 摘 要： | 目的：介绍一种全新的角膜内皮细胞培养方法。方法：在体视显微镜下撕下角膜后弹力层及内皮细胞层并剪切成小块，分置于培养皿中，将盖玻片叠加在组织块上进行培养。结果：角膜内皮细胞很快自组织块迁移生长，结论：本法培养内皮细胞不需用酶，具有简便，可靠，不易污染，细胞量大的优势，值得推广。
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| 关 键 词： | 角膜 内皮细胞 细胞培养 兔 |
| 文章编号： | 1001-7275(2001)03-0170-03 |
| 修稿时间： | 2001-03-15 |
New culture method of rabbit corneal endothelial cells |
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| YANG Peng ,LIU Yao ,ZENG Shui lin,ZHANG Hui bao. New culture method of rabbit corneal endothelial cells[J]. Journal of Southeast Univ: Medical Sci Ed, 2001, 20(3): 170-172 |
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| Authors: | YANG Peng LIU Yao ZENG Shui lin ZHANG Hui bao |
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| Affiliation: | YANG Peng 1,LIU Yao 2,ZENG Shui lin,ZHANG Hui bao 1 |
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| Abstract: | Objective To introduce a new method to culture corneal endothelial cells.Methods Through stero microscope,Descemet's membrane and endothelial cell layer were torn apart and cut out into small pieces,then the pieces were put into culture containers and coverglasses were piled up on tissue pieces for culture.Results The corneal endothelial cells were transformed quickly from tissue pieces.Conclusion Enzyme is not required in this method,moreover,the method has many advantages,such as simple,reliable,pollution proof and high yield.In a word,it is a good method worth recommending. |
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| Keywords: | corneal endothelial cell cell culture rabbits |
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