电针“足三里”穴对胃粘膜损伤大鼠血流量及一氧化氮、内皮素影响的实验研究

目的:探讨电针“足三里”对胃粘膜损伤大鼠保护作用的机制。方法:Wistar大鼠30只,雌雄各半,随机分为正常对照组、模型组、电针“足三里”组,每组10只。采用乙醇灌胃造成胃粘膜损伤大鼠模型,观察电针“足三里”对损伤指数(LI)、胃粘膜血流量(GMBF)、血清一氧化氮(NO)、血浆内皮素(ET)的影响。结果:胃粘膜损伤模型存在GMBF下降、LI升高、血清NO下降及血浆ET升高。电针“足三里”后可使NO回升,ET回降,GMBF亦明显增加,LI下降。结论:电针“足三里”对胃粘膜损伤起保护作用,其机制可能与调节血中NO、ET水平有很大关系。

Effects of Electroacupuncture of "Zusanli" (ST 36) on Gastric Mucosal Blood Flow, NO and ET Contents in Gastric Mucosal Injury Rats

Objective: To study the protective mechanism of electroacupuncture(EA) of "Zusanli"(ST 36) in improving gastric mucosa injury in rats.Methods: Thirty Wistar rats were randomized into control,model and EA groups with 10 cases in each group.Gastric mucosal injury model was made by intragastric perfusion of anhydrous alcohol(0.4 mL).EA(4/20 Hz in frequency,0.5 ms in pulse-duration,0-8 V instrength) was applied to bilateral "Zusanli"(ST 36) for(20 min).Gastric mucosal blood flow(GMBF) was detected with a blood flow detector,and gastric mucosal lesion index(GMLI) calculated according to Guth's criteria.Serum nitric oxide(NO) and plasma endothelin(ET) contents were assayed with nitrate reductase method and enzyme-linked immunoassay respectively.Results: Following perfusion of anhydrous alcohol,gastric mucosal lesion was very evident and GMBF decreased remarkably in model group(P<0.01),after EA,GMLI decreased considerably and GMBF increased significantly(P<0.01).Compared with control group,serum NO level decreased significantly and plasma ET increased considerably in model group(P<0.01);while compared with model group,serum NO increased significantly and plasma ET decreased significantly in EA group(P<0.01).Conclusion: EA of "Zusanli"(ST 36) can effectively relieve alcohol-induced gastric mucosal injury,which maybe be related to its regulative effects on blood NO and ET.