| 姜黄素诱导乳腺癌MCF-7细胞凋亡 |
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| 引用本文： | 韦达,唐金海,潘立群. 姜黄素诱导乳腺癌MCF-7细胞凋亡[J]. 江苏医药, 2008, 34(4): 348-351 |
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| 作者姓名： | 韦达 唐金海 潘立群 |
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| 作者单位： | 南京中医药大学,210029;江苏省肿瘤医院普通外科 |
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| 摘 要： | 目的 研究姜黄素对人乳腺癌细胞株MCF-7细胞增殖抑制和诱导凋亡作用.方法 MTT法检测姜黄素对MCF-7细胞的增殖抑制作用;流式细胞术(FCM)PI单染检测细胞周期;Annexin V/PI双染法检测细胞凋亡;Western blot法检测Bcl-2和Bax蛋白的表达.结果 姜黄素对MCF-7细胞生长有明显抑制作用,并呈剂量、时间依赖性;姜黄素能使MCF-7细胞阻滞在G1/S期,可以诱导细胞凋亡,Bax蛋白表达上调,而Bcl-2的表达减少.结论 姜黄素对人乳腺癌MCF-7细胞的增殖具有显著的抑制作用并可诱导细胞凋亡.其分子作用机制可能与其上调Bax基因表达水平的同时下调Bcl-2基因表达水平,从而诱导细胞凋亡有关.
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| 关 键 词： | 姜黄素 细胞凋亡 人乳腺癌细胞株MCF-7细胞 |
| 文章编号： | 0253-3685(2008)04-0348-03 |
| 修稿时间： | 2007-10-25 |
Apoptosis of breast cancer MCF-7 cells induced by curcumin |
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| WEI Da,TANG Jinhai,PAN Liqun. Apoptosis of breast cancer MCF-7 cells induced by curcumin[J]. Jiangsu Medical Journal, 2008, 34(4): 348-351 |
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| Authors: | WEI Da TANG Jinhai PAN Liqun |
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| Affiliation: | WEI Da,TANG Jinhai,PAN Liqun.Nanjing University of Traditional Chinese Medicine,Nanjing 210029,CHINA |
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| Abstract: | Objective To investigate the inhibiting effect of curcumin on the apoptosis of human breast cancer MCF-7 cells. Methods Growth inhibition rates of MCF-7 cells were measured with MTT method. The cell cycle distribution was detected by flow cytometry and cell apoptosis was detected by PI fluorescent staining.The expressions of Bcl-2 and Bax protein were detected by Western blot. Results Curcumin inhibited the proliferation of MCF-7 cells in a does-and time-dependent manner. The cell growth was arrested at G1/... |
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| Keywords: | Curcumin Apoptosis MCF-7 cells |
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