压力及维生素B1作用下视网膜神经细胞p53、MDM2及Ref1基因的表达

目的:探讨压力及压力加维生素B1联合作用下SD大鼠视网膜神经细胞p53、MDM2及Ref1基因的表达情况。方法:新生SD大鼠视网膜神经细胞体外培养,倒置相差显微镜观察细胞的生长情况,细胞分单纯加压组、实验加压组(加入维生素B1)及未加压对照组,于加压后2 d(培养第9~10天)行苔盼兰染色检查计数细胞成活率,p53免疫组化检查并提取细胞总RNA,用逆转录聚合酶链反应法检测p53、MDM2及Ref1基因的表达。结果:单纯压力组p53／MDM2基因的表达较正常未加压组增强,Ref1基因表达无增强;而维生素B1作用后的加压组,其p53／MDM2基因的表达较单纯加压组明显减弱。结论:压力作用下视网膜神经细胞的损害有凋亡机制参与,维生素B1对压力作用下视网膜神经细胞具有保护作用。

The Expression of p53、MDM2 and Ref1 Gene in Cultured Retina Neurons of SD Rats Treated with Vitamin B1 and/or Elevated Pressure

PURPOSE: To investigate the expression of p53, MDM2 and Ref1 gene in cultured retina neurons of SD rats treated with Vitamin B1 and (or) elevated pressure. METHODS: The retinal neuron of postnatal SD rats were cultured in vivo, the elevated pressure was produced after 7 days, and the total RNA was extracted after another 2 days, expression of p53, MDM2 and Ref1 gene were analyzed with RT-PCR. RESULTS: The expression level of p53 and MDM2 gene were increased in elevated pressure group, normal with Ref1 gene expression.But the expression of p53 and MDM2 gene were decreased significantly in elevated pressure group treated with vitamine B1 compare to the elevated group. CONCLUSION: Apoptosis seem to be a mechanism of cell death in retinal neurons of SD rats with elevated pressure.Vitamine B1 have protect effects against elevated pressure.