可移植性人脑胶质瘤组织裸小鼠脑内移植及其MR显像研究

背景与目的:文献报道的胶质瘤动物原位移植模型,大多数是将细胞悬液立体定向接种于鼠脑内,操作繁杂,耗时长,难以在短时间内完成批量实验.本研究接种可移植性人脑胶质瘤组织于裸小鼠脑内,探讨建立人脑胶质瘤裸小鼠原位移植模型及其MR活体成像的可行性.方法:在套管针内,置入位于裸小鼠皮下生长的可移植性人脑胶质瘤组织2 mm3,经微型颅钻钻颅孔后经套管针推入裸小鼠右尾状核内,第30天在配有小鼠专用micro-23微线圈的1.5T MR机上进行头颅扫描和专用软件测量显像的肿瘤体积.继而取全脑作连续冰冻切片,HE染色,在光学显微镜下观察肿瘤病理特征,在体视显微镜目镜下用测微尺测量肿瘤.然后将上述两种方法测得的数据进行统计学分析,评价荷瘤鼠活体MR显像计算肿瘤体积的可行性.结果:经MR扫描的15只颅内接种肿瘤的小鼠,有14只的尾状核区域见到肿瘤显像;在脑切片上,与MR像相同部位见到了肿瘤组织.经脑切片测得的肿瘤体积(23.19±10.18)mm3]和经MRI测得的肿瘤体积(23.45±11.64)mm3]比较差异无统计学意义(P＞0.05).肿瘤模型制作成功率为93%(14/15),肿瘤模型MR显像成功率为100%(14/14).结论:经套管针定量植入胶质瘤组织于裸小鼠尾状核,制作可移植性裸小鼠人脑胶质瘤原位模型,具有操作便捷、省时、便于批量制作、致瘤率高等优点.配有小鼠专用微型线圈的1.5T MR机可用于荷瘤裸小鼠的头颅成像、在活体上对移植瘤进行定位和体积测量等研究.

Transplantation and magnetic resonance imaging of transplantable human glioma tissue in the brain of nude mice

BACKGROUND & OBJECTIVE: In previous reports, orthotopic transplantation models of glioma were produced by injecting cell suspension into the brain of mice, which is complex, time-consuming, and nearly impossible to prepare in a large scale within a short period. This study was to establish human glioma orthotopic transplantation model in nude mice by transplanting tumor tissue in the brain, and investigate magnetic resonance imaging (MRI) of the transplanted tumors. METHODS: Human glioma cells were injected subcutaneously into nude mice to form human glioma. The transplantable human glioma tissues (2 mm3) were put into trocar and directly injected into the caudate nucleus of nude mice. Thirty days later, the tumors were detected by 1.5T superconduct MR machine with micro-23 coil and measured by Dicomworks (V1.35) software. Tumor morphology was observed under light microscope with HE staining. Tumor volume was measured under stereomicroscope. The feasibility of measuring tumor volume according to MRI data was evaluated. RESULTS: MRI showed that in the 15 nude mice received orthotopic transplantation in the caudate nucleus, 14 developed glioma. Under microscope, glioma tissues were found at the same sites as where MRI indicated. Tumor volume was (23.45+/-11.64) mm3 as measured by MRI and (23.19+/-10.18) mm3 as detected under stereomicroscope (P>0.05). The successful rate of tumor model preparation was 93% (14/15). The successful rate of tumor imaging by MRI was 100% (14/14). CONCLUSIONS: Tissue quantitative transplantation via trocar is simple, time-saving, and easy to construct tumor model in a large scale with high successful rate. The 1.5T MR machine with micro-23 coil can be used to observe tumor position and size of orthotopic transplantation models of human glioma in nude mice.