| 广东汉族人群复杂短串联重复SE33(ACTBP2)位点的多态性 |
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| 引用本文： | 孙宏钰,台运春,曾艳红,陆惠玲,陈丽娴,伍新尧. 广东汉族人群复杂短串联重复SE33(ACTBP2)位点的多态性[J]. 中山大学学报(医学科学版), 2004, 25(1): 55-58 |
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| 作者姓名： | 孙宏钰 台运春 曾艳红 陆惠玲 陈丽娴 伍新尧 |
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| 作者单位： | 中山大学法医物证学教研室,广东,广州,510080 |
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| 基金项目： | 国家"211"工程建设项目 |
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| 摘 要： | [目的]调查 SE33位点在广东汉族群体的扩增片段长度多态性.[方法]应用荧光标记的引物对 SE33位点进行 PCR扩增,采用 Prism ABI377 DNA自动测序仪分离 PCR产物,结合等位基因梯阶和分子量内标进行基因分型.[结果] 在 114名广东汉族无关个体中观察到了 35个等位基因, 87种基因型.个体识别能力( DP)值为 0.986 1,杂合度( H)为 0.912 3.[结论] 荧光标记 SE33扩增体系个体特异性强,识别力高,是个体识别和亲子鉴定的高效的检测体系.
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| 关 键 词： | 短串联重复 遗传多态性 |
| 文章编号： | 1672-3554(2004)01-0055-04 |
| 修稿时间： | 2003-09-16 |
Polymorphisms of a Complex STR Locus SE33 (ACTBP2) in Guangdong Han Population |
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| SUN Hong-yu,TAI Yun-chun,ZENG Yang-hong,LU Hui-ling,CHEN Li-xian,WU Xin-yao. Polymorphisms of a Complex STR Locus SE33 (ACTBP2) in Guangdong Han Population[J]. Journal of Sun Yatsen University(Medical Sciences), 2004, 25(1): 55-58 |
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| Authors: | SUN Hong-yu TAI Yun-chun ZENG Yang-hong LU Hui-ling CHEN Li-xian WU Xin-yao |
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| Abstract: | [Objective]The allele frequency distribution of a complex STR locus SE33 in Guangdong Han population was investigated. [Methods] Analysis was carried out using the fluorescent labeled primer and Prism ABI377 DNA sequencer and genotyping was made by comparing with allelic specific ladder and the internal lane standard. [Results]35 alleles and 87 genetypes were observed in 114 unrelated individuals. The power of discrimination and the heterozygosity value were 0. 986 1 and 0. 912 3, respectively. [Conclusion]SE33 locus is a highly informative and polymorphic STR and suitable for individual identification and paternity testing. |
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| Keywords: | SE33(ACTBP2) |
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