| 乙肝病毒前S1抗原时间分辨荧光免疫分析试剂盒的研制 |
| |
| 引用本文： | 胡妮娅,董志宁,周卓龙,李明,吴英松.乙肝病毒前S1抗原时间分辨荧光免疫分析试剂盒的研制[J].热带医学杂志,2009,9(5). |
| |
| 作者姓名： | 胡妮娅 董志宁 周卓龙 李明 吴英松 |
| |
| 作者单位： | 1. 南方医科大学生物技术学院,广州,510515
2. 广州市达瑞抗体工程技术有限公司,广州,510663 |
| |
| 基金项目： | 国家863项目(No.2006AA02A311) |
| |
| 摘 要： | 目的利用时间分辨荧光免疫分析(TRFIA)技术建立乙型肝炎病毒前S1抗原的检测试剂盒。方法应用双抗体夹心法建立乙肝preS1抗原TRFIA检测试剂盒,对试剂盒的各项性能指标进行评估。结果对280份血清样本进行检测并与国产酶联免疫法试剂盒对比,结果显示自制试剂盒的特异性更好;200份正常人血清样本检测结果表明,该试剂盒的cutoff值=阴性对照荧光值×4.5。用自制质控品检测分析内和分析间的精密度分别为3.4%～7.8%,6.9%～8.4%;检测HBsAg及HBeAg无交叉反应。结论试剂盒各项指标均达到临床检测要求,TRFIA法精密度和特异性有较大提高,可替代酶免法试剂盒。
|
| 关 键 词： | 乙肝preS1抗原 时间分辨荧光免疫分析（TRFIA） 检测 |
Development of A Time-Resolved Fluoroimmunoassay Kit for Detection of Hepatitis B Virus preS1 Antigen |
| |
| HU Ni-ya,DONG Zhi-ning,ZHOU Zhuo-long,LI Ming,WU Ying-song.Development of A Time-Resolved Fluoroimmunoassay Kit for Detection of Hepatitis B Virus preS1 Antigen[J].Journal Of Tropical Medicine,2009,9(5). |
| |
| Authors: | HU Ni-ya DONG Zhi-ning ZHOU Zhuo-long LI Ming WU Ying-song |
| |
| Institution: | 1.College of Biotechnology;the Southern Medical University;Guangdong;Guangzhou 510515;2.Guangzhou Darui Antibody Co.Ltd;Guangzhou 510663;China |
| |
| Abstract: | Objective To evaluate a home-made time-resolved fluoroimmunoassay(TRFIA) kit for the clinical detection of hepatitis B virus preS1 antigen.Methods An antibody-sandwich based TRFIA kit for developed for the detection of hepatitis B virus preS1 antigen.Results 280 serum samples were tested for hepatitis B virus preS1 antigen with the home-made TRFIA kit and the results were compared with the ELISA method.The home-made TRFIA kit was more specific than the ELISA method.According to the results from 200 normal s... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
