| 酸性成纤维细胞生长因子植物表达载体的构建及转化烟草的初步研究 |
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| 引用本文： | 蒋世翠,王义,李校堃,韩秀文,张美萍.酸性成纤维细胞生长因子植物表达载体的构建及转化烟草的初步研究[J].生物医学工程学杂志,2010(1). |
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| 作者姓名： | 蒋世翠 王义 李校堃 韩秀文 张美萍 |
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| 作者单位： | 吉林农业大学生命科学学院; |
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| 基金项目： | 十一五“863”计划生物反应器重大专项资助项目(2007AA100503); 吉林省科技发展重点计划资助项目(20070922); 高等学校科技创新工程重大项目培育基金资助项目(70S018); 长春市科技计划资助项目(06GG150) |
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| 摘 要： | 为了降低aFGF的生产成本,结合植物生物反应器的优点,就aFGF在转基因烟草中的表达进行了探索。将aFGF基因插入植物表达载体pBI121中,获得了含有aFGF基因的植物表达载体pBI121-TΩAB-aF,利用农杆菌介导法将基因转化烟草,转基因烟草在含有卡那霉素和头孢霉素的培养基培养,获得具有卡那抗性的转基因烟草植株,通过PCR检测、RT-PCR检测及Western blot检测证实外源基因已经在烟草中成功表达。为植物生物反应器生产aFGF提供了理论基础。
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| 关 键 词： | 酸性成纤维细胞生长因子 烟草 植物生物反应器 |
A Rudimentary Study of the Acid Fibroblast Growth Factor's Plant Expression Vector Construction and Transformation Tobacco |
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| Jiang Shicui Wang Yi Li Xiaokun Han Xiuwen Zhang Meiping.A Rudimentary Study of the Acid Fibroblast Growth Factor's Plant Expression Vector Construction and Transformation Tobacco[J].Journal of Biomedical Engineering,2010(1). |
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| Authors: | Jiang Shicui Wang Yi Li Xiaokun Han Xiuwen Zhang Meiping |
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| Institution: | Jiang Shicui Wang Yi Li Xiaokun Han Xiuwen Zhang Meiping(College of Life Sciences,Jilin Agricultere University,Changchun 130118,China) |
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| Abstract: | Acid fibroblast growth factor(aFGF) has great potential in clinical application,but it is very expensive.In order to reduce the cost of production and to make full use of the merits integrated with plant bioreator,we have explored the aFGF in transgenic Tobacco expression.AFGF gene was inserted into plant expression vector pBI121;the acquired plants contained aFGF gene expression vector pBI121-TOAB-aF.Using Agrobacterium-mediated gene transformation of Tobacco and using transgenic Tobacco containing kanamyc... |
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| Keywords: | Acid fibroblast growth factor(aFGF) Tobacco The plant bioreactor |
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