多发性硬化患者T细胞克隆中CD3CD56双阳性细胞的分析

目的多发性硬化(MS)是一种器官特异的自身免疫病,病变侵犯中枢神经系统.MS不仅有T,B细胞介导,而且与脑淋巴瘤的发生有密切关系.方法本文用T细胞克隆技术,细胞表型分析技术和细胞毒活性分析经MBP抗原肽治疗的MS患者外周血的T,B淋巴细胞(另文发表).结果经MBP抗原肽诱导的T细胞克隆中有相当一部分T细胞系不仅高表达CD3CD56双阳性标志(NKT细胞),50.7%±4.9%,对照为6.0%和3.0%,而其他神经系统疾病分别是11.0%和20.0%,且这种双阳性细胞群不同于NK细胞能特异杀伤神经胶质瘤(59.7%±4.9%)而NK细胞仅为17.3%±4.5%,具有统计学差异.结论用MBP抗原肽诱导的T细胞克隆可用作疫苗治疗MS患者,而这种高表达CD3CD56双阳性标志(NKT细胞)对神经胶质瘤细胞较NK细胞潜在的更强的杀伤活性.至于其抗瘤机制正在研究之中.

CD3/CD56 double positive cells in T cell lines generated from MS patients

Objective Multiple sclerosis (MS) is considered to be an organ-specific autoimmune disease in which the lesion mainly occurred in the central nervous system. It is believed that T and B cells are involved in the progress. Methods The phenotype and cytotoxicity of T cell lines setting up from MS patients who were treated with vaccine of alter MBP peptides and non--MS patients were analyzed. Results There were expression of CD3/CD56(NKT cell) 50.7% 4.9% of the T cell lines from MS patients induced by MBP peptides, and expression of the same marker, 6.0% and 3.0% of the T cell lines from health individual. As parallel study, there were 11.0% and 20.0% T cell lines expresing the CD3/CD56 in the T cell lines generating from non-MS inflammatory diseases of the central nervous diseases. The cytotokicity assay showed that NKT cells could kill the specific target Glioma cells, and the percent of cytotoxicity reached 59.7%, compared with the NK which kill the Glioma cells with significantly low percent, 17.3%. Conclusion The result indicated that the alter MBP vaccine-treated the MS patients could induce the MBP autoreactive T cell clones deletion and the NKT cells induced from the patients showed higher cytotoxic activity than NK cell. The antitumor machanism of the NKT is under in- vestigation.