葛根中异黄酮含量的薄层光密度法测定

本文报道了葛根中异黄酮成分含量的薄层光密度测定法。用甲苯—甲醇—10%甲酸(7:3:0.02)和乙酸乙酯—甲醇—50%甲酸(8:2:0.2)为展开剂,在硅胶G薄层上分离了大豆甙元、大豆甙、葛根素和大豆甙元-4′,7-二葡萄糖甙,并用CS-910双波长薄层扫描仪进行了定量测定。变异系数为1.5～1.6%,采用本法测定了生药和片剂样品的含量。

QUANTITATIVE TLC-DENSITOMETRY OF ISOFLAVONES IN PUERARIA LOBATA (WILLD.) OHWI

This paper reports a TLC-densitometric method to determine the isoflavones content in the Pueraria lobata (Willd.) Ohwi and its extract tablets. The developing solvent toluene-methanol-10% formic acid (7:3:0.02) was used for seperation of daidzein, daidzin and puerarin, and ethyl acetate-methanol-50% formic acid (8:2:0.2) for daidzein-4',7-diglucoside. The densitometric determination was carried out with a Shimadzu TLC-scanner CS-910. The parameter for the linearization of the working curves were: λ_s=250nm, λ_R=360nm, reflectance mode, zig-zag scanning, SX=3. The working curves were obtained, all passing through the origin. The four isoflavones content in Pueraria lobata (Willd.) Ohwi for two species from eight areas were determined. The coefficient of variation was 1.5～1.6%.