| Ag85A与Ag85BDNA疫苗联合免疫对可移植性鼠膀胱肿瘤的免疫调节效应 |
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| 引用本文： | 杨晓峰,张晓俊,王鹏.Ag85A与Ag85BDNA疫苗联合免疫对可移植性鼠膀胱肿瘤的免疫调节效应[J].现代泌尿外科杂志,2009,14(1):8-11. |
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| 作者姓名： | 杨晓峰 张晓俊 王鹏 |
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| 作者单位： | 山西医科大学第一医院泌尿外科,山西太原,030001 |
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| 摘 要： | 目的探讨联合应用Ag85A与Ag85BDNA疫苗抗膀胱肿瘤的免疫效应。方法采用小鼠膀胱癌BTT739细胞株，构建可移植性T739小鼠膀胱肿瘤模型。随机法分为6组：生理盐水组、BCG组、pcDNA3．1＋组、pcDNA—Ag85A组、pcDNA-Ag85B组及pcDNA—Ag85A和pcDNA-Ag85B联合组。荷瘤后第7天、14天和21天各肌注1次，末次注射后第7天检测各组小鼠脾脏的CD4＋和CD8＋T细胞亚群数量及CD4＋／CD8＋比值、小鼠血清IFN-γ、肿瘤体积重量并进行组织病理学检查。结果Ag85A与Ag85BDNA疫苗联合免疫组CD4＋和CD8＋T细胞亚群数量及CD4＋／CD8＋比值、血清IFN-γ明显高于DNA疫苗单独免疫组；小鼠的肿瘤重量明显低于DNA疫苗单独免疫组，差异有显著性（P〈0．05）；肿瘤组织病理学分析Ag85A与Ag85BDNA疫苗联合免疫组肿瘤坏死较DNA疫苗单独免疫组明显，且瘤体周围及瘤体内炎性细胞浸润较多。但Ag85A与Ag85BDNA疫苗联合、pcDNA—Ag85A、pcDNA—Ag85BDNA疫苗单独应用组均不及单纯应用BCG的免疫调节效应。结论Ag85A与Ag85BDNA疫苗联合免疫在抗膀胱肿瘤过程中具有明显的免疫效应，明显优于DNA疫苗单独免疫，二者联用具有协同增强免疫效应；但Ag85A与Ag85BDNA疫苗联合免疫效应尚达不到BCG的作用。
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| 关 键 词： | 膀胱癌 卡介苗 DNA疫苗 Ag85A Ag85B |
The immunological effect of Ag85A and Ag85B DNA vaccine on rat bladder cancer |
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| YANG Xiao-feng,ZHANG Xiao-jun,WANG Peng.The immunological effect of Ag85A and Ag85B DNA vaccine on rat bladder cancer[J].Journal of MOdern Urology,2009,14(1):8-11. |
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| Authors: | YANG Xiao-feng ZHANG Xiao-jun WANG Peng |
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| Institution: | Department of Urology;the First Hospital;Shanxi Medical University;Taiyuan 030001;China |
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| Abstract: | Objective To explore the immunological effect of the DNA vaccine encoding mycobacterium tuberculosis Ag85A with Ag85B in mice with bladder cancer. Methods The mice with bladder cancer were constructed by injecting mice bladder cancer cell strain BTT739 subcutaneously,which were then divided randomly into six groups:NS,BCG,pcDNA3.1,pcDNA-Ag85A,pcDNA-Ag85B,and pcDNA-Ag85A+ pcDNA-Ag85B groups. The mice were injected subcutaneously on the 7th,14th,and 21st day respectively. After 7 days,the number of CD4+ and C... |
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| Keywords: | Ag85A Ag85B |
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