| 儿童感染的乙型肝炎病毒S基因的分子特征 |
| |
| 引用本文： | 周艳,刘发娣,刘金辉,王鹏,熊英,龚甜.儿童感染的乙型肝炎病毒S基因的分子特征[J].南昌大学学报(医学版),2011,51(6). |
| |
| 作者姓名： | 周艳 刘发娣 刘金辉 王鹏 熊英 龚甜 |
| |
| 作者单位： | 南昌大学研究生院医学部;江西省儿童医院检验科;南昌大学医学院微生物学教研室;江西省疾病预防控制中心疾病检测所; |
| |
| 摘 要： | 目的初步调查儿童感染的乙型肝炎病毒表面抗原的基因特征。方法收集10名HBV感染的儿童血清标本,抽提血清中的HBV DNA,采用PCR扩增HBV S基因并测序,利用Genotyping软件对PCR产物序列进行分型,并分析HBV S基因的突变情况。结果 10份血清标本中,扩增出HBV S基因并成功测序6份,其余4份扩增阴性不满足测序要求。6份PCR产物所测序列标本经Genotyping比对后,均属于B型,与NCBI收录的参考序列AF100309同源性最高;5例是adw,1例是ayw。HBV S基因中编码a抗原决定簇的核苷酸序列突变点分别为G529A、T531C、C534A、T562A、T581A、A589C。编码a抗原决定簇的氨基酸序列突变点分别为I126T、P127T、S143T、G145A。核苷酸突变点G529A、T562A不引起编码a抗原决定簇的氨基酸序列突变,为无义突变。而其他4点突变均可引起编码a抗原决定簇相应氨基酸序列发生改变。结论所调查儿童主要感染B基因型HBV;其感染的HBV S抗原a决定簇的氨基酸序列出现I126T、P127T、S143T、G145A的突变,可能是逃避乙肝疫苗诱导的免疫保护的一种...
|
| 关 键 词： | 乙型肝炎病毒 “a”抗原决定簇 基因突变 儿童 |
Characteristics of S Gene of Hepatitis B Virus in Infected Children |
| |
| ZHOU Yana,LIU Fa-di,LIU Jin-huib,WANG Pengc,XIONG Ying,GONG Tian.Characteristics of S Gene of Hepatitis B Virus in Infected Children[J].Journal of Nanchang University(Medical Science),2011,51(6). |
| |
| Authors: | ZHOU Yana LIU Fa-di LIU Jin-huib WANG Pengc XIONG Ying GONG Tian |
| |
| Institution: | ZHOU Yan1a,LIU Fa-di2,LIU Jin-hui1b,WANG Peng1c,XIONG Ying3,GONG Tian3(1a.2009 Grade of Medical Department of Graduate School,1b.Department of Microbiology of Medical College,1c.2008 Grade of MedicalDepartment of Graduate School,Nanchang University,Nanchang 330006,China,2.Department of Clinical Laboratory,Jiangxi Children's Hospital,3.Department of Detection of the Disease,Jiangxi Centers for Disease Control and Prevention,Nanchang 330029,China) |
| |
| Abstract: | Objective To investigate the gene characteristics of hepatitis B virus surface antigen(HBsAg) in infected children.Methods Serum samples were collected from 10 children infected with hepatitis B virus(HBV),and HBV DNA was extracted for S gene amplification by PCR.The PCR products were sequenced and compared with standard sequence,and the genotype was analyzed using on-line genotyping.Results Among the 10 serum samples,S gene was amplified and sequenced in 6 samples,and the other 4 samples did not meet seque... |
| |
| Keywords: | hepatitis B virus a determinant gene mutation child |
| 本文献已被 CNKI 等数据库收录！ |
|
