| TSA抑制人胰腺癌PANC1细胞生长的实验研究 |
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| 引用本文： | 汪理|周伟|张景辉|刘涛|王春友.TSA抑制人胰腺癌PANC1细胞生长的实验研究[J].中国普通外科杂志,2010,19(3):234-238. |
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| 作者姓名： | 汪理|周伟|张景辉|刘涛|王春友 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院胰腺外科中心; |
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| 基金项目： | 国家自然科学基金资助项目(30801098) |
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| 摘 要： | 目的研究去乙酰化转移酶抑制剂TSA对人胰腺癌PANC-1细胞生长的影响及其作用机制。方法用不同浓度的TSA处理PANC-1细胞。MTT法检测肿瘤细胞的增殖;流式细胞仪分析细胞凋亡及细胞周期;Western免疫印迹及荧光定量RT-PCR分析抗凋亡基因bcl-2和肿瘤抑制基因p21WAF1/CIP1的表达。结果TSA作用于PANC-1细胞能明显抑制细胞的增殖,且具有明显的剂量依赖性;TSA处理72 h的PANC-1细胞其早期凋亡率明显提高(P0.05),G0/G1期细胞比例显著升高(P0.05),S期细胞比例显著降低(P0.05)。TSA在转录和翻译水平上能明显下调bcl-2的表达(P0.05),上调p21WAF1/CIP1的表达(P0.05)。结论TSA可以通过诱导胰腺癌细胞的凋亡和周期阻滞而抑制癌细胞生长;其发生机制可能与下调抗凋亡基因bcl-2和上调肿瘤抑制基因p21WAF1/CIP1的表达有关。
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| 关 键 词： | 胰腺肿瘤 组蛋白酶乙酰化酶抑制剂 bcl-2 p21 WAF1/CIP1 |
| 收稿时间： | 2009-08-05 |
| 修稿时间： | 2009-10-30 |
Trichostatin A (TSA) suppresses the growth of pancreatic adenocarcinoma cells |
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| WANG Li,ZHOU Wei,ZHANG Jinghui,LIU Tao,WANG Chunyou.Trichostatin A (TSA) suppresses the growth of pancreatic adenocarcinoma cells[J].Chinese Journal of General Surgery,2010,19(3):234-238. |
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| Authors: | WANG Li ZHOU Wei ZHANG Jinghui LIU Tao WANG Chunyou |
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| Institution: | WANG Li,ZHOU Wei,ZHANG Jinghui,LIU Tao,WANG Chunyou(Department of Pancreatic Surgery,Union Hospital,Huazhong University of Science , Technology,Wuhan 430022,China) |
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| Abstract: | Objective To investigate the effects of histone deacetylase inhibitor trichostatin A(TSA),on the cell growth of human pancreatic cancer cell line PANC-1 in vitro and its mechanism.Methods The proliferating activity of PANC-1 cells was observed by MTT assay.The cell apoptosis and cell cycle distribution were detected by flow cytometry.Western blot and real-time PCR were used to assess the expression levels of inhibitor of apoptosis gene bcl-2 and cell cycle regulation gene p21WAF1/CIP1.Results A dose depende... |
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| Keywords: | Pancreatic Neoplasms Histone Deacetylase Inhibitor bcl-2 p21WAF1/CIP1 |
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