同型半胱氨酸致血管内皮细胞DNA损伤的作用

[目的]探讨同型半胱氨酸（HCY）对血管内皮细胞的DNA损伤作用。[方法]体外培养鼠主动脉内皮细胞，随机分为正常对照组和HCY9种浓度组，MTT法检测细胞毒作用，彗星试验检测DNA损伤情况，取培养液测定丙二醛（MDA）含量和超氧化物歧化酶（SOD）活力。[结果]（1）对照组细胞呈单层鹅卵石样，紧密排列。1、2．5、5mmol／L HCY组细胞无明显变化，而自10mmol／L起，HCY组细胞排列杂乱，聚集成团。MTT法显示细胞生长明显受抑制，抑制率达20％；（2）彗星试验显示自5mmol／L HCY起尾长、尾矩和Olive尾矩明显增加；（3）自10mmol／L起HCY培养液中MDA含量高于对照组，SOD活力低于对照组。[结论]HCY具有明显的细胞毒作用，其机制可能与HCY直接造成DNA损伤和促进氧化应激有关

The Effects of DNA Damage on Vascular Endothelial Cells Impaired by Homocysteine

[Objective] To study the effect of DNA damage on vascular endothelial cells impaired by homocysteine.[Methods] Vascular endothelial cells were cultured and treated with 9 homocysteine concentrations.Cultured cells were examined by MTT colorimetric assay,DNA damage was observed by single cell gel electrophoresis assay(comet assay),MDA contents and SOD activity were measured.[Results](1) Homocysteine had an inhibitory effect on the proliferation and morphology of vascular endothelial cells since 10 mmol/L con...