亚低温联合盐酸法舒地尔对创伤性脑损伤后Bcl-2和Bax表达的影响

目的：探讨亚低温联合脑血管扩张药盐酸法舒地尔对脑外伤（TBI）大鼠脑细胞Bcl-2、Bax蛋白表达的影响及其对外伤后受损害脑组织的保护作用。方法：30只SD大鼠随机分为假手术组（Sham）、创伤性脑损伤组（TBI）、亚低温治疗组（MHT）、法舒地尔治疗组（FSD）及亚低温联合法舒地尔组（MHT＋FSD），每组6只。Allen＇s改良法制作海马细胞凋亡的模型，予以亚低温及亚低温联合法舒地尔治疗。HE染色观察海马细胞形态学的变化；免疫组化法检测神经细胞Bcl-2和Bax蛋白表达的变化。结果：（1）HE染色。MHT组与FSD组海马细胞均较TBI组形态规则，胞核浓染、固缩的现象明显减少。与MHT组及FSD组相比，MHT＋FSD组细胞层次更多，排列也更为整齐。（2）免疫组化检测。与TBI组相比，MHT组及FSD组Bcl-2蛋白达表水平显著增高（P〈0．05），Bax的表达明显降低（P〈0．05）；与MHT组及FSD组相比，MHT＋FSD组Bcl-2蛋白表达显著增强（P〈0．05），Bax的表达显著降低（P〈0．05）。结论：亚低温联合法舒地尔治疗可通过有效抑制TBI后细胞凋亡而发挥脑保护作用。

Effects of Mild Hypothermia and Fasudil on Expression of Bcl-2 and Bax in Rats after Traumatic Brain-injury

Objective：To investigate effects of mild hypothermia （MHT） combined with fasudil （MHT ＋ FSD） on expression of Bcl -2 and Bax in rats after traumatic brain injury （TBI）, and explore this therapeutic regimen protection for brain tissue against TBI. Methods：Thirty SD rats were randomly divided into five groups： sham group, TBI group, MHT group, FSD group and MHT ＋ FSD group （n = 6）. The models of hippocampus cell apoptosis in rats were established by improved Allen＇s method. HE staining was used to observe the changes of cell morphology, immuno - histochemical staining to detect expression of Bcl-2 and Bax. Resuits：HE staining showed that the hippocampus cells in MHT and FSD groups presented more regular appearance with dense nucleus and fewer karyopycnosis than those in MHT ＋ FSD group, moreover, more cellular layers and well arranged cells were observed in MHT ＋ FSD group. The immunohistochemistry staining demonstrated that the expression of Bcl-2 was significantly stronger and Bax expression was markedly lower in hippocampus CA3 region in MHT ＋ FSD group than that in MHT and FSD groups. Conclusions ： Combination therapy of mild hypothermia and fasudil may strengthen the brain protection through inhibition of cell apoptosis following traumatic brain injury.