Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet |
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| Authors: | Elke Ueberham Jan Böttger Uwe Ueberham Jens Grosche Rolf Gebhardt |
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| Institution: | (1) Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany;(2) Department for Molecular and Cellular Mechanisms of Neurodegeneration, Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany;(3) Department for Pathophysiology of Neuroglia, Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany;(4) Interdisciplinary Centre for Clinical Research, Medical Faculty of the University of Leipzig, Leipzig, Germany |
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| Abstract: | Background Proliferation of oval cells, the bipotent precursor cells of the liver, requires impeded proliferation and loss of hepatocytes
as well as a specific micro-environment, provided by adjacent sinusoidal cells of liver. Despite their immense importance
for triggering the oval cell response, cells of hepatic sinusoids are rarely investigated. To elucidate the response of sinusoidal
liver cells we have employed a choline-deficient, ethionine-supplemented (CDE) diet, a common method for inducing an oval
cell response in rodent liver. We have utilised selected expression markers commonly used in the past for phenotypic discrimination
of oval cells and sinusoidal cells: cytokeratin, E-cadherin and M2-pyruvate kinase for oval cells; and glial fibrillary acidic
protein (GFAP) was used for hepatic stellate cells (HSCs). |
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