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Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diagnosing hydatidosis |
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| Authors: | Elham Kalantari Mojgan Bandehpour Ramin Pazoki Niloofar Taghipoor-Lailabadi Hooshang Khazan Nariman Mosaffa Mohammad Reza Nazaripouya Bahram Kazemi |
| Institution: | (1) Department of parasitology and mycology, Shahid Beheshti University, M.C, Tehran, Iran;(2) Cellular and Molecular Biology Research Center, Shahid Beheshti University, M.C, Tehran, Iran;(3) Microbiology Department, Semnan University of Medical Sciences, Semnan, Iran;(4) Department of Immunology, Shahid Beheshti University, M.C, Tehran, Iran; |
| Abstract: | Echinococcus granulosus causes human cystic echinococcosis as an important public health problem in many regions of the world. There are some problems in primary diagnosis such as cross-reaction with sera from patients with other parasitic disease in serological tests. The use of an appropriate source of antigenic material is a very important and crucial point in the improvement of the serodiagnostic features such as enzyme-linked immunosorbent assay (ELISA) method. We expressed and purified recombinant AgB of Echinococcus granulosus and used as antigen in ELISA method. Serum samples were given from 36 cystic hydatid disease patients that have been confirmed by surgical operation as well as 36 healthy individuals sera were tested by ELISA method using recombinant AgB and compared with commercial kit (Euroimmun) for specificity and sensitivities value. The sensitivity of 91.66% and specificity of 97.22% were determined by homemade kit. |
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