慢性肾功能不全患者外周血血小板P-选择素、L-选择素和细胞间粘附分子-1表达的研究

目的 :研究血小板P -选择素 (CD6 2 -P)、淋巴细胞L -选择素 (CD6 2 -L)和细胞间粘附因子 - 1(ICAM- 1)在慢性肾功能不全时的变化规律及与肾功能的相关性。方法 :用流式细胞术及免疫荧光染色法 ,分析了4 9例慢性肾功能不全患者外周血血小板P -选择素 (CD6 2 -P) ,淋巴细胞L -选择素 (CD6 2 -L) ,ICAM - 1(CD - 5 9)表达情况。结果 :慢性肾功能不全各期患者外周淋巴细胞L -选择素表达量与正常对照组无显著性差异 (P >0 .0 5 ) ,氮质血症期和尿毒症期患者血小板P -选择素表达量低于正常对照组 (P <0 .0 5 ) ,而尿毒症期ICAM - 1表达量高于正常对照组 (P <0 .0 5 )。患者CD6 2 -P及ICAM - 1与肾功能指标血尿素氮(BUN)及血肌肝 (Cr)之间有明显的的相关性 (P >0 .0 5 )。结论 :血小板P选择素、ICAM - 1表达可能与慢性肾功能损坏的进程有关。

EXPRESSION OF P-SELECTIN ON PLATELET AND L-SELECTIN, ICAM-1 ON LYMPHOCYTES FROM PATIENTS WITH CHRONIC RENAL INSUFFICIENT

Objective:To study the change and correlation of P-selection, L-selection and ICAM-1 in chronic renal insufficient. Methods: Adhesion molecule P-selection(CD62P) on platelet and L-selection, ICAM-1 on lymphocytes from peripheral blood were investigated in 49 patients with chronic renal failure by using flow cytometric analysis and immunofluorescence staining method.Result:There was not significantly difference between L-selection of chronic renal insufficient and that of normal central(P>0.05).Expression of P-selection of patients whose remaining kidney function was not replaced was decreased comdpared with normal central (P<0.05). Otherwise expression of ICAM-1 of uremia stage was increased compared with normal central (P<0.05).Conclusion:The study suggests that P-selectin and ICAM-1 expression may predict the progression of the destroying of renal function.