甘肃地区人群雌激素受体α-29位基因多态性与HBV感染转归的关系

目的了解甘肃地区人群雌激素受体ERα-29位多态性与HBV感染后果的关系,从基因水平上探讨慢性乙型肝炎(CHB)的发病机制。方法选取2011年8月-2012年5月在本院就诊的102例CHB患者、106例HBV感染自愈者采用聚合酶链反应-限制性片段长度多态性法检测其雌激素受体ERα-29位多态性。用基因计数法计算检验人群的等位基因频率,进行Hardy-Weinberg遗传平衡定律检验。应用非条件Logistic回归分析将混杂因素进行调整。基因型及等位基因频率比较采用行×列的χ2检验。结果 CHB患者雌激素受体基因ERα-29位多态性的TT基因型和T等位基因频率(62.7%、82.4%)明显高于HBV感染恢复期患者(48.1%、69.8%),差异有统计学意义(χ2=4.502,P0.05;χ2=4.478,P0.05);CC基因型和C等位基因频率(12.8%、17.6%)明显低于后者(19.8%、30.2%),差异有统计学意义(χ2=27.219,P0.05;χ2=4.478,P0.05);T等位基因HBV感染慢性化的风险是C等位基因的2.018倍(OR=2.018,95%CI:1.047~3.891)。结论雌激素受体ERα-29位TT基因型和T等位基因可能是HBV感染慢性化的遗传易感基因。

Relationship between estrogen receptor alpha gene polymorphisms at position 29 and outcome of hepatitis B virus in- fection among population in Gansu,China

ObjectiveTo investigate the relationship between estrogen receptor alpha （ERα） gene polymorphisms at position 29 and the outcome of hepatitis B virus （HBV） infection among the population in Gansu, China, and to explore the pathogenesis of chronic hepatitis B （CHB） at the genetic level. MethodsA total of 102 patients with CHB and 106 patients who recovered from HBV infection were included in the study. The ERα gene polymorphisms at position 29 were analyzed by polymerase chain reaction-restriction fragment length polymorphism. The allele frequency was calculated by the gene counting method, and the Hardy-Weinberg equilibrium test was performed. Confounding factors were subjected to unconditional logistic regression analysis. Comparisons of genotype frequency and allele frequency were made by chi-square test （R×C）. ResultsThe frequencies of TT genotype and T allele of ERα gene polymorphisms at position 29 were significantly higher in the CHB patients than in the patients in the recovery period of HBV infection （62.7% vs 48.1%, χ2=4.502, P〈0.05; 82.4% vs 69.8%, χ2=4.478, P〈0.05）, while the frequencies of CC genotype and C allele were significantly lower in the former than in the latter （12.8% vs 19.8%, χ2=27.219, P〈0.05; 17.6% vs 30.2%, χ2=4.478, P〈0.05）. The risk of chronic HBV infection in patients with T allele was 2.018 times that in patients with C allele （OR = 2.018, CI = 1.047-3.891）. ConclusionThe ERα gene with TT genotype at position 29 might be the susceptibility gene for chronic HBV infection.